College of Pharmaceutical Sciences, Southwest University, Chongqing, 400716, P. R. China.
Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing, Technology and Health, College of Chemistry, Central China Normal University, Wuhan, 430079, P. R. China.
Chemistry. 2020 Jun 18;26(34):7583-7588. doi: 10.1002/chem.202002330. Epub 2020 May 28.
Co single-atom catalysts (SACs) with good aqueous solubility and abundant labelling functional groups were prepared in Co/Fe bimetallic metal-organic frameworks by a facile solvothermal method without high-temperature calcination. In contrast to traditional chemiluminescence (CL) catalysts, Co SACs accelerated decomposition of H O to produce a large amount of singlet oxygen ( O ) rather than superoxide (O ) and hydroxyl radical (OH ). They were found to dramatically enhance the CL emission of the luminol-H O reaction by 1349 times, and, therefore, were employed as very sensitive signal probes for conducting CL immunoassay of cardiac troponin I. The detection limit of the target analyte was as low as 3.3 pg mL . It is the first time that employment of SACs for boosting CL reactions has been validated. The Co SACs can also be employed to trace other biorecognition events with high sensitivity.
通过简便的溶剂热法在 Co/Fe 双金属金属有机骨架中制备了具有良好水溶性和丰富标记官能团的单原子催化剂 (SACs)，而无需高温煅烧。与传统的化学发光 (CL) 催化剂相比，Co SACs 加速了 H 2 O 2 的分解，产生了大量的单线态氧 ( 1 O 2 )，而不是超氧阴离子 (O 2 - ) 和羟自由基 (OH )。研究发现，它们使鲁米诺-H 2 O 2 反应的 CL 发射显著增强了 1349 倍，因此可用作心肌肌钙蛋白 I 的 CL 免疫分析的非常灵敏的信号探针。目标分析物的检测限低至 3.3 pg·mL -1 。这是首次验证 SACs 用于增强 CL 反应。Co SACs 还可用于高灵敏度地追踪其他生物识别事件。
