Yamada M, Kawaguchi M, Takamiya H, Wada H, Okigaki T
Division of Cell Biology, Shigei Medical Research Institute, Okayama, Japan.
Cell Struct Funct. 1988 Dec;13(6):495-513. doi: 10.1247/csf.13.495.
An epithelial cell line, designated as SGE1, has been established from isolated rat renal glomeruli. SGE1 cells are able to grow continuously in a serum-free medium at similar growth rates to those in the medium containing serum. Quantitative studies of the cells in the serum-free condition demonstrated that SGE1 cells required a collagen type I or IV, fibronectin, or laminin-coated substratum for adhesion and growth, and among them, collagen type I and IV were most effective. Essential medium supplements for the adhesion and growth were epidermal growth factor and transferrin, respectively, and both effects were noticeably enhanced with linoleic acid. Morphological observation found that in a monolayer culture, SGE1 cells formed domes, and in a collagen embedding culture, they formed cystic spheres having features of a simple cuboidal epithelium, polarized formation of microvilli and tight junctions as well as a lateral cell membrane with cytoplasmic projections. In addition, SGE1 cells expressed Fx1A antigens, which are nephritogenic antigens on their microvilli.
一种名为SGE1的上皮细胞系已从分离的大鼠肾小囊中建立。SGE1细胞能够在无血清培养基中以与含血清培养基中相似的生长速率持续生长。对无血清条件下细胞的定量研究表明，SGE1细胞需要I型或IV型胶原、纤连蛋白或层粘连蛋白包被的基质用于黏附和生长，其中I型和IV型胶原最为有效。黏附和生长所需的基本培养基补充物分别是表皮生长因子和转铁蛋白，且这两种作用在亚油酸存在时均显著增强。形态学观察发现，在单层培养中，SGE1细胞形成穹顶状，在胶原包埋培养中，它们形成具有单层立方上皮特征、微绒毛和紧密连接的极化形成以及带有细胞质突起的侧细胞膜的囊性球体。此外，SGE1细胞在其微绒毛上表达Fx1A抗原，这是一种致肾炎抗原。
