College of Marine Science and Biological Engineering, Qingdao University of Science & Technology, Qingdao, 266042, China; Key Laboratory for Biochemical Engineering of Shandong Province, Qingdao, 266042, China.
College of Marine Science and Biological Engineering, Qingdao University of Science & Technology, Qingdao, 266042, China.
Toxicon. 2020 Jul 30;182:59-65. doi: 10.1016/j.toxicon.2020.05.005. Epub 2020 May 16.
This study evaluated the performance of an easy-cultivation device for the mass culture of Alexandrium minutum (A. minutum), a dinoflagellate that produces paralytic shellfish toxins (PSTs). Five culture conditions including three different sizes of containers (250 mL conical flask, 500 mL beaker, and 20 L jar) in two different environments (out-incubator and incubator) were compared in terms of growth and PSTs production. Compared with the incubator environment, the out-incubator environment had more fluctuations in temperature and light intensity. Results showed that the cell densities of A. minutum increased in all groups, especially in the conical flask (I, out-incubator, 6.29×10 cells/mL) and the beaker (IV, incubator, 7.28×10 cells/mL). When cultured in the 20 L jar under out-incubator condition, the algae had the lowest cell density (2.82×10 cells/mL) but the highest toxicity (93.42 ± 2.55×10 MU/cell). The negative correlation between average growth rate and single-cell toxicity could be explained by biocompatibility, thereby indicating that the low growth rate led to high toxicity. HPLC-FLD showed that the cellular toxicity increased due to the quantitative increase in GTX1/4, which are the more toxic derivatives. The PSTs types consistently contained GTX1/4 and GTX2/3. The differences in algae growth and toxin-production could be due to changes in bacteria (out-incubator) and CO (incubator) with vessel size. The effects of environmental factors on algae are strain specific. The out-incubator device can be applied for large-scale cultivation of A. minutum considering the algae density and toxin-producing ability.
本研究评估了一种易于培养的亚历山大藻（Alexandrium minutum，A. minutum）大规模培养装置的性能，亚历山大藻是一种产生麻痹性贝类毒素（PSTs）的甲藻。在两种不同环境（外孵育器和孵育器）下，比较了包括三种不同容器大小（250 mL 锥形瓶、500 mL 烧杯和 20 L 罐）在内的五种培养条件，以评估其生长和 PSTs 产生情况。与孵育器环境相比，外孵育器环境的温度和光照强度波动更大。结果表明，A. minutum 的细胞密度在所有组中均增加，尤其是在锥形瓶（I，外孵育器，6.29×10 细胞/mL）和烧杯（IV，孵育器，7.28×10 细胞/mL）中。在 20 L 罐中于外孵育器条件下培养时，藻类的细胞密度最低（2.82×10 细胞/mL），但毒性最高（93.42±2.55×10 MU/细胞）。平均生长率与单细胞毒性之间的负相关关系可以用生物相容性来解释，表明低生长率导致高毒性。HPLC-FLD 表明，由于 GTX1/4 的定量增加，细胞毒性增加，GTX1/4 是毒性更高的衍生物。细胞毒性增加。毒素类型始终包含 GTX1/4 和 GTX2/3。藻类生长和毒素产生的差异可能是由于容器尺寸变化导致的细菌（外孵育器）和 CO（孵育器）变化所致。环境因素对藻类的影响具有菌株特异性。考虑到藻类密度和产毒能力，外孵育器装置可用于亚历山大藻的大规模培养。
