Yang Xihong, Hu Xiaoqun, Dong Zequn, Li Min, Zheng Zuoxing, Xie Wancui
College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Shandong, Qingdao, 266042, China; Shandong Provincial Key Laboratory of Biochemical Engineering, Shandong, Qingdao, 266042, China.
College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Shandong, Qingdao, 266042, China.
Toxicon. 2021 Jun;196:1-7. doi: 10.1016/j.toxicon.2021.03.006. Epub 2021 Mar 12.
Economic bivalve ingested toxic algae causes frequent human poisoning events. To explore new compounds that can accelerate the depuration of toxins in shellfish, we investigated the detoxification of the paralytic shellfish toxins (PSTs) and the biotransformation pathway of PSTs during detoxification by the application of three treatments to a toxic bloom, Alexandrium minutum (A. minutum). The detoxification effect of Platymonas subcordiformis (PS) mixed with carboxymethyl chitosan (CMC) group is significantly better than the starving group in each oyster tissues. The toxicity of viscera which occupied 78.95% of total toxicity reduced to 155 MU/100g after 13 days' depuration experiment. And adding CMC could significantly achieve rapid detoxification and effectively reduce the STX to 0.07 μmol/100 g in viscera. Meanwhile, PSTs underwent biotransformation during the depuration process, which mainly manifested as GTX1/4→GTX2/3→STX, GTX2→dcGTX2. This study explored a new strategy for toxin depuration in shellfish.
经济双壳贝类摄入有毒藻类会引发频繁的人类中毒事件。为探索能加速贝类毒素净化的新化合物，我们通过对有毒藻华微小亚历山大藻（A. minutum）进行三种处理，研究了麻痹性贝类毒素（PSTs）的解毒作用以及解毒过程中PSTs的生物转化途径。亚心形扁藻（PS）与羧甲基壳聚糖（CMC）混合组在各牡蛎组织中的解毒效果显著优于饥饿组。经过13天的净化实验，占总毒性78.95%的内脏毒性降至155 MU/100g。添加CMC可显著实现快速解毒，并有效将内脏中的石房蛤毒素（STX）降至0.07 μmol/100g。同时，PSTs在净化过程中发生了生物转化，主要表现为GTX1/4→GTX2/3→STX、GTX2→dcGTX2。本研究探索了一种贝类毒素净化的新策略。
