State Key Laboratory of Phytochemistry and Plant Resources in West China and Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China and Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Fitoterapia. 2020 Sep;145:104629. doi: 10.1016/j.fitote.2020.104629. Epub 2020 May 16.
Based on a method combining the LC-MS/MS molecular networking strategy with the conventional means of phytochemical research, the chemical constituents and the availability of Paris tengchongensis, a new species found in 2017 from Yunnan Province, were investigated for the first time. The molecular networking showed that this species contained the characteristic steroidal glycosides of the genus Paris by comparison of those of Paris polyphylla var. yunnanensis. Furthermore, the detailed investigation on the 80% EtOH extract of its rhizomes resulted to the isolation of twenty steroidal glycosides including three new spirostane-type saponins, named paristengosides A-C (1-3). Their structures were confirmed by spectroscopic analyses (HRMS and NMR) and chemical methods. The new isolates were evaluated for their cytotoxicities against two human cancer cell lines (HEL and MDA-MB-231), anti-inflammatory effects on a lipopolysaccharide (LPS)-stimulated NO production model in RAW264.7 macrophages, anti-AChE, and antimicrobial activities. The results from the molecular networking and the investigation on the chemical constituents suggested that P. tengchongensis can be used as a potential resource of Rhizoma Paridis.
基于一种结合 LC-MS/MS 分子网络策略与传统植物化学研究手段的方法，首次对 2017 年在云南省发现的新物种云南球兰进行了化学成分和可用性研究。分子网络比较表明，该物种含有云南重楼特征性甾体糖苷。此外，对其根茎 80%EtOH 提取物的详细研究导致分离出二十种甾体糖苷，包括三种新的螺甾烷型皂苷，命名为球兰苷 A-C（1-3）。它们的结构通过光谱分析（高分辨质谱和 NMR）和化学方法确定。新分离物的细胞毒性对两种人癌细胞系（HEL 和 MDA-MB-231）、对 LPS 刺激 RAW264.7 巨噬细胞中 NO 产生模型的抗炎作用、对 AChE 的抑制作用和抗菌活性进行了评估。分子网络和化学成分研究的结果表明，云南球兰可作为重楼的潜在资源。
