Crizel R L, Perin E C, Siebeneichler T J, Borowski J M, Messias R S, Rombaldi C V, Galli V
Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Plant Physiol Biochem. 2020 May 11;152:211-220. doi: 10.1016/j.plaphy.2020.05.003.
Strawberry is one of the most popular fruits because of its sensorial and functional properties. However, strawberry crop is sensitive to salt stress conditions. Despite plants have plasticity, high salt concentrations induce molecular, biochemical, and physiological responses in plants. There is evidence that the abscisic acid (ABA) hormone acts as a signaling molecule under stress conditions; however, the molecular mechanisms involved in the synthesis and homeostasis of ABA and in the induction of phytochemical antioxidant compounds under stress conditions remain unclear. In this study, the effect of stress induced by NaCl (salt stress - SS), with or without the simultaneous application of ABA, on the ABA, phenylpropanoids and L-ascorbic acid (AsA) metabolisms were evaluated. The physiological aspects (Na, Cl and proline concentration, photosynthetic variables) were also studied. The results showed that salt stress increases the Na and Cl content in the leaves, affects photosynthetic variables and triggers the production of proline, pelargonidin-3-O-glucoside, total phenolic compounds and AsA content, alongside the upregulation of several genes from the phenylpropanoid and flavonoid pathways. These effects were accompanied by the induction of compounds and transcripts related to ABA biosynthesis, conjugation and catabolism. Otherwise, the exogenous application of ABA in salt stressed plants promotes a shift in gene expression and metabolism to mitigate the stress. Therefore, salt stress affects the metabolism of ABA, phenylpropanoids and AsA in strawberry increasing phytochemical composition which is strongly associated with an ABA-dependent mechanism.
草莓因其感官特性和功能特性而成为最受欢迎的水果之一。然而，草莓作物对盐胁迫条件敏感。尽管植物具有可塑性，但高盐浓度会诱导植物产生分子、生化和生理反应。有证据表明，脱落酸（ABA）激素在胁迫条件下起信号分子的作用；然而，胁迫条件下ABA的合成与稳态以及植物化学抗氧化化合物诱导过程中涉及的分子机制仍不清楚。在本研究中，评估了NaCl诱导的胁迫（盐胁迫 - SS）在有或没有同时施用ABA的情况下对ABA、苯丙烷类化合物和L-抗坏血酸（AsA）代谢的影响。还研究了生理方面（钠、氯和脯氨酸浓度、光合变量）。结果表明，盐胁迫会增加叶片中的钠和氯含量，影响光合变量，并引发脯氨酸、天竺葵素-3-O-葡萄糖苷、总酚类化合物和AsA含量的产生，同时上调苯丙烷类和类黄酮途径中的几个基因。这些影响伴随着与ABA生物合成、共轭和分解代谢相关的化合物和转录本的诱导。此外，在盐胁迫植物中外源施用ABA会促进基因表达和代谢的转变以减轻胁迫。因此，盐胁迫会影响草莓中ABA、苯丙烷类化合物和AsA的代谢，增加植物化学成分，这与ABA依赖机制密切相关。
