Wang Renyuan, Yang Xijia, Chi Yaowei, Zhang Xia, Ma Xianzhong, Zhang Dan, Zhao Ting, Ren Yongfeng, Yang Haiyan, Ding Wenjiang, Chu Shaohua, Zhou Pei
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Affairs, Shanghai, China.
Front Plant Sci. 2024 Nov 21;15:1497362. doi: 10.3389/fpls.2024.1497362. eCollection 2024.
Salt stress could lead to plant growth barriers and crop yield reduction. Strawberries are sensitive to salt stress, and improving salt tolerance is important for strawberry production. This study aimed to explore the potential of hydrogen-rich water (HRW) to enhance salt tolerance in strawberries. Through pot experiments, we investigated how HRW affects plant growth, ion absorption, osmotic stress, oxidative stress, antioxidant enzyme levels, hormone levels, and root endophytic bacteria in strawberry seedlings under salt stress. The results showed that under 100 mM NaCl treatment, 50% and 100% HRW treatments significantly increased strawberry biomass by 0.29 g and 0.54g, respectively, wherein, 100% HRW significantly increased the shoot and root length by 15.34% and 24.49%, respectively. In addition, under salt stress the absorption of K by strawberry seedlings was increased with the HRW supplement, while the absorption of Na was reduced. Meanwhile, HRW treatment reduced the transfer of Na from root to shoot. Furthermore, under salt stress, HRW treatment increased the relative water content (RWC) by 12.35%, decreased the electrolyte leakage rate (EL) by 7.56%. HRW modulated phytohormone levels in strawberry seedlings, thereby alleviating the salt stress on strawberries. Moreover, HRW was found to promote plant growth by altering the diversity of bacteria in strawberry roots and recruiting specific microorganisms, such as . Our findings indicate that HRW could help restore the microecological homeostasis of strawberry seedlings, thus further mitigating salt stress. This study provides a novel perspective on the mechanisms by which HRW alleviates salt stress, thereby enriching the scientific understanding of hydrogen's applications in agriculture.
盐胁迫会导致植物生长受阻和作物减产。草莓对盐胁迫敏感，提高耐盐性对草莓生产至关重要。本研究旨在探讨富氢水（HRW）提高草莓耐盐性的潜力。通过盆栽试验，我们研究了HRW如何影响盐胁迫下草莓幼苗的植物生长、离子吸收、渗透胁迫、氧化胁迫、抗氧化酶水平、激素水平和根内生细菌。结果表明，在100 mM NaCl处理下，50%和100% HRW处理分别使草莓生物量显著增加0.29 g和0.54 g，其中100% HRW使地上部和根部长度分别显著增加15.34%和24.49%。此外，在盐胁迫下，补充HRW可增加草莓幼苗对K的吸收，同时减少对Na的吸收。同时，HRW处理减少了Na从根向地上部的转运。此外，在盐胁迫下，HRW处理使相对含水量（RWC）提高了12.35%，电解质渗漏率（EL）降低了7.56%。HRW调节了草莓幼苗中的植物激素水平，从而减轻了对草莓的盐胁迫。此外，发现HRW通过改变草莓根际细菌的多样性和招募特定微生物（如……）来促进植物生长。我们的研究结果表明，HRW有助于恢复草莓幼苗的微生态稳态，从而进一步减轻盐胁迫。本研究为HRW缓解盐胁迫的机制提供了新的视角，从而丰富了对氢在农业中应用的科学认识。
