干眼病证治小方潜在有效成分的研究。

Study on the potential effective ingredients of Xiaosheng prescription for dry eye disease.

机构信息

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, China.

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.

出版信息

Biomed Pharmacother. 2020 Jul;127:110051. doi: 10.1016/j.biopha.2020.110051. Epub 2020 May 16.

DOI:10.1016/j.biopha.2020.110051

PMID:32428832

Abstract

Xiaosheng prescription (XSP) has been used for dry eye disease (DED) for more than 10 years in Eye Hospital of China Academy of Chinese Medicine Sciences. However, the effective ingredients involved have remained unclear, which was investigated in this study by the correlation of ingredient and therapeutic activity. Human corneal epithelial cells (HCEC) cultured with 110 mM NaCl solution in vitro and C57BL/6 mice injected subcutaneously with scopolamine hydrobromide were used to establish dry eye models, and the therapeutic effect of XSP extract 1 was better than that of XSP extract 2 significantly. Then, UPLC-Q-TOF/MS and data analysis program Progenesis QI and Makerlynx XS were used to analyze the potential effective ingredients of XSP, and 4 compounds were speculated and identified, in which Schisandrin and 1 μM of Schisantherin A could obviously increase the cell survival rate of injured cells on the cell model. It can be indicated that Schisandrin and Schisantherin A are probably the potential effective ingredients in XSP for DED.

摘要

消生方（XSP）在 10 多年来一直被用于中国中医科学院眼科医院的干眼病（DED）治疗中。然而，其涉及的有效成分仍不清楚，本研究通过成分与治疗活性的相关性进行了调查。体外使用 110mM NaCl 溶液培养人角膜上皮细胞（HCEC）和皮下注射氢溴酸东莨菪碱的 C57BL/6 小鼠被用于建立干眼症模型，XSP 提取物 1 的治疗效果明显优于 XSP 提取物 2。然后，使用 UPLC-Q-TOF/MS 和数据分析程序 Progenesis QI 和 Makerlynx XS 分析 XSP 的潜在有效成分，推测并鉴定出 4 种化合物，其中五味子素和 1μM 的五味子丙素可以明显提高细胞模型中受损细胞的细胞存活率。这表明五味子素和五味子丙素可能是 XSP 治疗 DED 的潜在有效成分。