Kuznetsova Marina V, Gizatullina Julia S, Nesterova Larisa Yu, Starčič Erjavec Marjanca
Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, Goleva street 13, 614081 Perm, Russia.
Department of Biology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
Microorganisms. 2020 May 15;8(5):741. doi: 10.3390/microorganisms8050741.
strains isolated from case of colibacillosis in Russian poultry farms in the region of Perm Krai were analyzed for their sensitivity to main antibiotics and bacteriocins. Sensitivity profiles for 9 antibiotics and 20 bacteriocins were determined with the disc diffusion method and the overlay test, respectively. Further, with the PCR the presence of several and integron 1 genes was revealed and the phylogenetic group for each strain determined. Among the 28 studied strains 85.7% were resistant to at least three antibiotics, 53.6% to five or more drugs, and 10.7% to eight antibiotics. PCR revealed that the gene was harbored by 71.4% of strains and the gene by 53.6% of strains. The class 1 integrons were found in 28.6% of strains. All of the studied strains were insensitive to ten or more bacteriocins. More than 90% of the studied strains were insensitive to pore-forming colicins of group A and B colicins, while 60.7% were insensitive to colicins with DNase and RNase activity. All of the analyzed strains were insensitive to at least two of the tested microcins. Neither the antibiotic resistance profile nor the bacteriocin resistance profile correlated with phylogenetic group of the strains. Thus, the studied strains were shown to possess high levels of multiple resistance to antibiotics and insensitivity to bacteriocins.
对从俄罗斯彼尔姆边疆区家禽养殖场大肠杆菌病病例中分离出的菌株进行了主要抗生素和细菌素敏感性分析。分别采用纸片扩散法和覆盖试验测定了9种抗生素和20种细菌素的敏感性谱。此外，通过聚合酶链反应（PCR）揭示了几种基因和整合子1基因的存在，并确定了每个菌株的系统发育群。在所研究的28株大肠杆菌中，85.7%对至少三种抗生素耐药，53.6%对五种或更多药物耐药，10.7%对八种抗生素耐药。PCR显示，71.4%的菌株携带基因，53.6%的菌株携带基因。在28.6%的菌株中发现了1类整合子。所有研究菌株对十种或更多细菌素不敏感。超过90%的研究菌株对A组成孔大肠杆菌素和B组大肠杆菌素不敏感，而60.7%的菌株对具有DNA酶和RNA酶活性的大肠杆菌素不敏感。所有分析菌株对至少两种测试的微菌素不敏感。抗生素耐药谱和细菌素耐药谱均与菌株的系统发育群无关。因此，所研究的菌株显示出对抗生素的高水平多重耐药性和对细菌素的不敏感性。
