Eid Samah, Tolba Hala M N, Hamed Rehab I, Al-Atfeehy Nayera M
Department of Bacteriology, Reference Laboratory for Veterinary Quality Control on Poultry Production (RLQP), Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), P.O. Box 264-Dokki, Nadi El-Seidst., Giza 12618, Egypt.
Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
Saudi J Biol Sci. 2022 May;29(5):3380-3389. doi: 10.1016/j.sjbs.2022.02.015. Epub 2022 Feb 17.
Avian pathogenic (APEC) is considered a severe issue to both poultry business and health of the general public. In that context, 50 samples from 250 diseased broiler chickens in 10 chicken farms were employed to isolation. Microbiological techniques were employed to detect isolates of from 250 diseased broiler chickens which were examined by antimicrobial susceptibility profiles against 11 antimicrobial agents using disc diffusion technique as well as their biofilm forming capacity were detected. In addition to, study the isolation and purification of phages based on spot technique to verify that lytic phages are present in isolates and plaque assay for titration of bacteriophages. In the present research, we also looked at the ability of bacteriophages to inhibit and dissolve previously formed biofilms by O78 isolate. Moreover, experimental testing of O78 bacteriophages for colibacillosis prevention and control in one day old broiler chicks were done. The obtained results showed that twenty-six isolates out of 50 examined samples were isolated (10.4%). The most prevalent serotypes were O78, O121:H7, O146:H2, O124, O113:H4, O112:H2, O1:H7, O55:H7, O2:H6, O91:H21, O26:11. Antibiogram results demonstrated the resistance of isolates with high percentage 100% were against, Ampicillin, Amoxicillin and Tetracycline. Biofilm quantification analysis showed that 24/26 (92.3%) isolates were considered biofilm producer isolates. The characterization and the lytic activity of bacteriophage were performed based on Transmission electron microscopy and showed the greatest lytic activity against the evaluated host strains with effective activity at concentration of 10 at 24 h and strong significant reduction of the established O 78 biofilm within 12 h. The result of experimental infection showed that the performance indicators of phage in treated and challenged group showed high significant increase in body weight, weight gain and improved FCR than infected -antibiotic treated and infected bacteriophage and antibiotic treated. Total viable cell counts of in the lungs of birds revealed that there is highly significant difference between the six groups count results. We concluded that phage therapy found to be an attractive option to prevent and control multidrug resistant colibacillosis in broilers.
禽致病性大肠杆菌（APEC）被认为对家禽业和公众健康都是一个严重问题。在此背景下，从10个养鸡场的250只患病肉鸡中采集了50份样本用于分离。采用微生物技术从250只患病肉鸡中检测分离株，使用纸片扩散法对其进行针对11种抗菌剂的药敏试验，并检测其生物膜形成能力。此外，基于斑点技术研究噬菌体的分离和纯化，以验证裂解性噬菌体是否存在于分离株中，并通过噬菌斑测定法对噬菌体进行滴定。在本研究中，我们还研究了噬菌体抑制和溶解O78分离株先前形成的生物膜的能力。此外，还对O78噬菌体在1日龄肉鸡雏鸡中预防和控制大肠杆菌病进行了实验测试。获得的结果表明，在50份检测样本中分离出26株分离株（10.4%）。最常见血清型为O78、O121:H7、O146:H2、O124、O113:H4、O112:H2、O1:H7、O55:H7、O2:H6、O91:H21、O26:11。药敏结果表明，分离株对氨苄西林、阿莫西林和四环素的耐药率高达100%。生物膜定量分析表明，24/26（92.3%）的分离株被认为是生物膜产生菌。基于透射电子显微镜对噬菌体的特性和裂解活性进行了研究，结果表明其对评估的宿主菌株具有最大的裂解活性，在浓度为10时24小时具有有效活性，并且在12小时内可显著减少已形成的O78生物膜。实验感染结果表明，噬菌体处理和攻毒组的性能指标显示，与感染抗生素处理组、感染噬菌体和抗生素处理组相比，体重、体重增加和饲料转化率均有显著提高。鸟类肺部的总活菌数显示，六组计数结果之间存在高度显著差异。我们得出结论，噬菌体疗法被认为是预防和控制肉鸡多重耐药大肠杆菌病的一个有吸引力的选择。
