Quiñones-Pérez Carlota, Martínez Amparo, Crespo Francisco, Vega-Pla José Luis
Laboratorio de Investigación Aplicada, Cría Caballar de las Fuerzas Armadas, Carretera de Madrid Km 395A, 14014 Córdoba, Spain.
Genetics Department, University of Córdoba, edificio Gregor Mendel (C-5), Campus de Rabanales, 14071 Córdoba, Spain.
Animals (Basel). 2020 May 17;10(5):868. doi: 10.3390/ani10050868.
Contagious equine metritis is receiving renewed attention due to the continuous detection of carriers in apparent agent-free farms. Interactions of with the seminal microflora may be the plausible cause behind these spontaneous changes of the carrier state. Accordingly, the aim of this study was to compare the differences in the seminal microbiome composition of one stallion in the contagious equine metritis carrier state and non-carrier state. Samples were cryopreserved after their extraction. Cell disruption was performed by high-speed homogenization in grinding media. Bacterial families were identified via V3 amplification of the 16S rRNA gene and Ion Torrent sequencing. Only bacterial families with relative abundance above 5% were taken into consideration. The positive sample contained a strong dominance of Corynebacteriaceae (37.75%) and Peptoniphilaceae (28.56%). In the negative sample, the Porphyromonadaceae (20.51%), Bacteroidaceae (19.25%) and Peptoniphilaceae (18.57%) families prevailed. In conclusion, the microbiome seminal composition varies when an individual carries from when it is free of it. The wider differences were found in the Corynebacteriaceae, Porphyromonadaceae and Bacteroidaceae families. Due to the limitations of a single-case analysis, further studies are needed for a better understanding of the stallion seminal microflora interactions.
由于在表面上无病原体的马场中持续检测到携带者，传染性马子宫内膜炎再次受到关注。[病原体名称]与精液微生物群的相互作用可能是携带者状态这些自发变化背后的合理原因。因此，本研究的目的是比较一匹处于传染性马子宫内膜炎携带者状态和非携带者状态种公马精液微生物组组成的差异。样本提取后进行冷冻保存。通过在研磨介质中高速匀浆进行细胞破碎。通过16S rRNA基因的V3扩增和Ion Torrent测序鉴定细菌科。仅考虑相对丰度高于5%的细菌科。阳性样本中棒杆菌科（37.75%）和消化链球菌科（28.56%）占主导地位。在阴性样本中，卟啉单胞菌科（20.51%）、拟杆菌科（19.25%）和消化链球菌科（18.57%）占优势。总之，个体携带[病原体名称]时与未携带时精液微生物组组成不同。在棒杆菌科、卟啉单胞菌科和拟杆菌科中发现了更广泛的差异。由于单病例分析的局限性，需要进一步研究以更好地了解种公马精液微生物群的相互作用。
