Clinical Microbiology and Parasitology Unit, Polyclinic 'Dr. Zora Profozić', Zagreb, Croatia.
Department of Biomedical Sciences, University Centre Varaždin, University North, Varaždin, Croatia.
Andrology. 2018 Jan;6(1):223-229. doi: 10.1111/andr.12453. Epub 2017 Dec 7.
Corynebacterium glucuronolyticum (C. glucuronolyticum) is a rare isolate that is only recently being acknowledged as a potential urogenital pathogen. The bibliographical references on this bacterial species are scarce, and its influence on all semen parameters was hitherto unknown - therefore, the aim of this study was to evaluate its effects on a range of sperm quality parameters. A prospective approach to compare semen parameters before and after treatment was used in this study. C. glucuronolyticum in semen specimens was identified using analytical profile index biotyping system (API Coryne) and additionally confirmed by matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS), with the determination of antimicrobial susceptibility by Kirby-Bauer method. Semen analysis was performed according to the criteria from the World Health Organization (with the use of Tygerberg method of sperm morphology categorization). Very strict inclusion criteria for participants also included detailed medical history and urological evaluation. From a total of 2169 screened semen specimens, the inclusion rate for participants with C. glucuronolyticum that satisfied all the criteria was 1.01%. Antibiogram-guided treatment of the infection with ensuing microbiological clearance has shown that the resolution of the infection correlates with statistically significant improvement in the vitality of spermatozoa, but also with a lower number of neck and mid-piece defects. Parameters such as sperm count, motility and normal morphology were not affected. In addition, susceptibility testing revealed a trend towards ciprofloxacin resistance, which is something that should be considered when selecting an optimal treatment approach. Albeit it is rarely encountered as a monoisolate in significant quantities, C. glucuronolyticum may negatively influence certain sperm parameters; therefore, it has to be taken into account in the microbiological analysis of urogenital samples.
解糖糖普雷沃菌（Corynebacterium glucuronolyticum，C. glucuronolyticum）是一种罕见的分离株，直到最近才被认为是一种潜在的泌尿生殖道病原体。关于这种细菌的文献参考很少，其对所有精液参数的影响也尚不清楚-因此，本研究旨在评估其对一系列精子质量参数的影响。本研究采用前瞻性方法比较治疗前后的精液参数。使用分析概况指数生物分型系统（API Coryne）鉴定精液标本中的 C. glucuronolyticum，并通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）进一步确认，通过 Kirby-Bauer 法测定抗菌药物敏感性。精液分析按照世界卫生组织的标准进行（使用 Tygerberg 精子形态分类法）。对参与者的纳入标准非常严格，包括详细的病史和泌尿科评估。在总共 2169 份筛选的精液标本中，符合所有标准的携带 C. glucuronolyticum 的参与者的纳入率为 1.01%。感染的抗生素图谱引导治疗并随后进行微生物学清除显示，感染的解决与精子活力的统计学显著改善相关，但也与颈部和中段缺陷的数量减少相关。精子计数、活力和正常形态等参数不受影响。此外，药敏试验显示出对环丙沙星耐药的趋势，在选择最佳治疗方法时应考虑到这一点。尽管它很少以显著数量的单菌定植出现，但解糖糖普雷沃菌可能会对某些精子参数产生负面影响；因此，在泌尿生殖道样本的微生物分析中必须考虑到这一点。
