Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Molecular Biology and Genetics, Gene Expression and Gene Medicine, Aarhus University, Aarhus, Denmark.
Iran Biomed J. 2020 Sep;24(5):288-94. doi: 10.29252/ibj.24.5.283. Epub 2020 May 2.
Previous data have shown the tumorigenicity roles of histone deacetylase 8 (HDAC 8) in breast cancer. More recently, the oncogenic effects of this molecule have been revealed in triple negative breast cancer (TNBC). The present study aimed to determine the diagnostic value of HDAC8 for the differentiation of TNBC from nTNBC tumors.
A total of 50 cancerous and normal adjacent tumor specimens were obtained, and the clinical and pathological findings of studied subjects were recorded. The expression of HDAC8 gene was determined by qRT-PCR. Also, immunohistochemical staining was performed on tissue samples.
Our results showed that the expression of HDAC8 in breast cancer tissues was significantly higher than the normal adjacent tissues (p = 0.0011). HDAC8 expression was also observed to be higher in TNBC patients than nTNBC group (p = 0.0013). In addition, in the TNBC group, there was a significant association between the HDAC8 overexpression and tumor characteristics, including tumor size (p = 0.039), lymphatic invasion (p = 0.01), tumor grade (p = 0.02), and perineural invasion (p < 0.05). The cut-off value was fixed at 0.6279 r.u., and the corresponding sensitivity and specificity were found to be 73.91% and 70.37%, respectively.
According to the findings, among the other markers, HDAC8 oncogene may be used as a potential tumor marker in the diagnosis of TNBC tumors.
先前的数据表明组蛋白去乙酰化酶 8（HDAC8）在乳腺癌中具有致瘤作用。最近，该分子的致癌作用已在三阴性乳腺癌（TNBC）中被揭示。本研究旨在确定 HDAC8 对区分 TNBC 与非 TNBC 肿瘤的诊断价值。
共获得 50 例癌组织和正常相邻肿瘤标本，记录研究对象的临床和病理发现。通过 qRT-PCR 测定 HDAC8 基因的表达。还对组织样本进行了免疫组织化学染色。
我们的结果表明，HDAC8 在乳腺癌组织中的表达明显高于正常相邻组织（p = 0.0011）。HDAC8 在 TNBC 患者中的表达也高于 nTNBC 组（p = 0.0013）。此外，在 TNBC 组中，HDAC8 过表达与肿瘤特征之间存在显著关联，包括肿瘤大小（p = 0.039）、淋巴浸润（p = 0.01）、肿瘤分级（p = 0.02）和神经周围浸润（p < 0.05）。将截止值固定在 0.6279 r.u.，对应的灵敏度和特异性分别为 73.91%和 70.37%。
根据这些发现，在其他标志物中，HDAC8 癌基因可能作为 TNBC 肿瘤诊断的潜在肿瘤标志物。
