Soucek Ondrej, Kacerovsky Marian, Musilova Ivana, Pliskova Lenka, Bolehovska Radka, Andrys Ctirad
Department of Clinical Immunology and Allergy, University Hospital Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
J Matern Fetal Neonatal Med. 2022 May;35(9):1703-1711. doi: 10.1080/14767058.2020.1767578. Epub 2020 May 19.
CD11b is an integrin molecule located on the surface of leukocytes. CD11b is involved in the processes of cell adhesion and migration. Expression of CD11b increases during inflammation. Therefore, this study was aimed at the evaluation of concentrations of CD11b in the amniotic fluid from pregnancies complicated by preterm prelabor rupture of the membranes (PPROM), with respect to the presence of microbial invasion of the amniotic cavity (MIAC), intra-amniotic inflammation (IAI), and microbial-associated IAI (the presence of both MIAC and IAI).
Eighty women with singleton pregnancies complicated by PPROM were included. Amniotic fluid samples were obtained by transabdominal amniocentesis. Amniotic fluid CD11b concentrations were determined by enzyme-linked immunosorbent assay. MIAC was determined by a non-cultivation approach. IAI was defined by a bedside amniotic fluid interleukin-6 concentration ≥745 pg/mL.
Women with MIAC or microbial-associated IAI had higher CD11b concentrations in the amniotic fluid than women without these complications (with MIAC: median 0.31 ng/mL versus without MIAC: median 0.17 ng/mL, = .001; with microbial associated-IAI: median 0.35 ng/mL versus without microbial-associated IAI: median 0.16 ng/mL; =.02). The presence of IAI was not associated with elevated CD11b concentrations. A weak negative correlation was found between amniotic fluid CD11b concentrations and interleukin-6 concentrations (rho = 0.26; = .02).
MIAC and microbial-associated IAI are characterized by higher amniotic fluid CD11b concentrations in pregnancies complicated by PPROM.
CD11b是一种位于白细胞表面的整合素分子。CD11b参与细胞黏附和迁移过程。在炎症过程中，CD11b的表达会增加。因此，本研究旨在评估胎膜早破（PPROM）合并妊娠的羊水样本中CD11b的浓度，同时考虑羊膜腔微生物入侵（MIAC）、羊膜腔内炎症（IAI）以及微生物相关的IAI（MIAC和IAI同时存在）情况。
纳入80例单胎妊娠合并PPROM的女性。通过经腹羊膜腔穿刺获取羊水样本。采用酶联免疫吸附测定法测定羊水CD11b浓度。采用非培养方法测定MIAC。IAI定义为床边羊水白细胞介素-6浓度≥745 pg/mL。
与未发生这些并发症的女性相比，发生MIAC或微生物相关IAI的女性羊水CD11b浓度更高（发生MIAC者：中位数0.31 ng/mL，未发生MIAC者：中位数0.17 ng/mL，P = 0.001；发生微生物相关IAI者：中位数0.35 ng/mL，未发生微生物相关IAI者：中位数0.16 ng/mL，P = 0.02）。IAI的存在与CD11b浓度升高无关。羊水CD11b浓度与白细胞介素-6浓度之间存在弱负相关（rho = 0.26，P = 0.02）。
在PPROM合并妊娠中，MIAC和微生物相关IAI的特点是羊水CD11b浓度较高。
