Sadeghi H, da Silva A, Klein C
E.A. Doisy Department of Biochemistry, St. Louis University Medical School, MO 63104.
Dev Genet. 1988;9(4-5):561-7. doi: 10.1002/dvg.1020090432.
117 antigen is involved in the process of intercellular cohesion in Dictyostelium discoideum [Brodie et al., 1983]. The antigen, a 69- and 72-kDa doublet, was found to arise from a 60- and 62-kDa precursor. The mature antigen contains N-linked oligosaccharides that are sulfated and fucosylated [Sadeghi et al., 1987]. These oligosaccharide chains are resistant to endoglycosidase H digestion. 117 antigen also contains a post-translationally added carbohydrate-containing modification(s). Unlike the N-linked oligosaccharide, this carbohydrate moiety is sensitive to periodate oxidation. 117 antigen is developmentally regulated, and the changes in rate of 117 antigen synthesis reflect changes in the cellular levels of its mRNA. 117 mRNA accumulates in starving cells and reaches its maximum when cells become aggregation competent. The mRNA levels then decline, and by the time the slug structure is formed, no 117 mRNA is present. 117 mRNA reaccumulates for a brief period during early culmination and then returns to an undetectable level.
117抗原参与了盘基网柄菌细胞间黏附过程[布罗迪等人，1983年]。该抗原为69 kDa和72 kDa的双峰，由60 kDa和62 kDa的前体产生。成熟抗原含有硫酸化和岩藻糖基化的N-连接寡糖[萨德吉等人，1987年]。这些寡糖链对内切糖苷酶H消化具有抗性。117抗原还含有翻译后添加的含碳水化合物修饰。与N-连接寡糖不同，这种碳水化合物部分对高碘酸盐氧化敏感。117抗原受发育调控，其合成速率的变化反映了其mRNA细胞水平的变化。117 mRNA在饥饿细胞中积累，当细胞具备聚集能力时达到最大值。然后mRNA水平下降，到蛞蝓结构形成时，已不存在117 mRNA。在早期发育阶段，117 mRNA会短暂重新积累，然后又回到检测不到的水平。
