Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Giza, Egypt.
Pathology Department, Faculty of Medicine, Cairo University, Giza, Egypt.
Biotechnol Lett. 2020 Aug;42(8):1597-1610. doi: 10.1007/s10529-020-02908-y. Epub 2020 May 19.
The aim of the current study was to evaluate the therapeutic and regenerative effects of MSCs derived exosomes in the treatment of type 1 DM and to compare its effects with MSCs themselves. The experiment was done on forty albino rats grouped as follows, group (1): Ten healthy rats, group (2): Ten induced type 1 DM rats, group (3): Ten induced type 1 DM rats received exosomes intraperitoneally, and group (4): Ten induced type 1 DM rats received MSCs intraperitoneally. Serum glucose and plasma insulin levels were assessed weekly. QRT-PCR was done to assess regeneration of pancreatic beta cells by measuring insulin, Pdx1, Smad2, Smad3 and TGFβ genes. Additionally, histopathological and immune-histochemical examinations were done to confirm pancreatic tissue regeneration.
Regarding the assessed genes (insulin, Pdx1, Smad2, Smad3 and Tgfβ) gene expression in MSCs treated group showed significant increase compared to diabetic group (p value < 0.001) and gene expression in exosomes treated group was increased significantly compared to diabetic and MSCs treated groups (p value < 0.001). Histopathological and immune-histochemical examination revealed regeneration of pancreatic islets in both treated groups.
MSCs Derived exosomes showed superior therapeutic and regenerative results than MSCs themselves.
本研究旨在评估间充质干细胞衍生的外泌体在 1 型糖尿病治疗中的治疗和再生效果，并将其与间充质干细胞本身的效果进行比较。实验在 40 只白化大鼠中进行，分为以下几组：第 1 组：10 只健康大鼠；第 2 组：10 只诱导的 1 型糖尿病大鼠；第 3 组：10 只诱导的 1 型糖尿病大鼠腹腔内注射外泌体；第 4 组：10 只诱导的 1 型糖尿病大鼠腹腔内注射间充质干细胞。每周评估血清葡萄糖和血浆胰岛素水平。通过测量胰岛素、Pdx1、Smad2、Smad3 和 TGFβ 基因，进行 QRT-PCR 评估胰腺β细胞的再生情况。此外，进行组织病理学和免疫组织化学检查以确认胰腺组织再生。
关于评估的基因（胰岛素、Pdx1、Smad2、Smad3 和 Tgfβ），间充质干细胞治疗组的基因表达与糖尿病组相比显著增加（p 值 < 0.001），外泌体治疗组的基因表达与糖尿病组和间充质干细胞治疗组相比显著增加（p 值 < 0.001）。组织病理学和免疫组织化学检查显示两组均有胰岛再生。
与间充质干细胞相比，间充质干细胞衍生的外泌体显示出更好的治疗和再生效果。
