A 组链球菌的全基因组序列分析与群体基因组学研究。

Whole Genome Sequence Analysis and Population Genomics of Group A Streptococci.

机构信息

Doherty Department, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.

Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, VIC, Australia.

出版信息

Methods Mol Biol. 2020;2136:81-111. doi: 10.1007/978-1-0716-0467-0_7.

DOI:10.1007/978-1-0716-0467-0_7

PMID:32430815

Abstract

Whole-genome sequencing (WGS) is used to determine the genetic composition of an organism. This fast-moving field is continually evolving through technical advancements and the development of new bioinformatic tools for analyzing genomic data; however, the basic principles and processes for defining and processing high-quality genome sequence information remain unchanged. Here, we introduce some considerations and describe some commonly used bioinformatic steps for processing raw genome sequence data to generate genome assemblies through to understanding basic population genomics.

摘要

全基因组测序（WGS）用于确定生物体的遗传组成。这个快速发展的领域通过技术进步和开发新的生物信息学工具来分析基因组数据不断发展；然而，定义和处理高质量基因组序列信息的基本原理和流程保持不变。在这里，我们介绍一些需要考虑的因素，并描述一些常用的生物信息学步骤，用于处理原始基因组序列数据，生成基因组组装，直至理解基本的群体基因组学。