Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy.
Methods Mol Biol. 2021;2278:31-44. doi: 10.1007/978-1-0716-1274-3_4.
Genome assembly and annotation are two of the key actions that must be undertaken in order to explore the genomic repertoire of (bifido)bacteria. The gathered information can be employed to genomically characterize a given microorganism, and can also be used to perform comparative genome analysis by including other sequenced (bifido)bacterial strains. Here, we highlight various bioinformatic programs able to manage next generation sequencing data starting from the assembly of a genome to the comparative analyses between strains.
基因组组装和注释是探索（双歧）细菌基因组库必须进行的两项关键操作。收集到的信息可用于对特定微生物进行基因组特征分析，也可用于通过包含其他测序的（双歧）细菌菌株来进行比较基因组分析。在这里，我们重点介绍了各种生物信息学程序，这些程序能够从基因组组装开始，对来自不同菌株的下一代测序数据进行管理，从而进行比较分析。
