Tsai Catherine Jia-Yun, Loh Jacelyn M S, Proft Thomas
Department of Molecular Medicine and Pathology, School of Medical Sciences and Maurice Wilkins Centre for Biomolecular Discovery, The University of Auckland, Auckland, New Zealand.
Methods Mol Biol. 2020;2136:279-286. doi: 10.1007/978-1-0716-0467-0_21.
Recently, the use of Galleria mellonella larvae as a nonmammalian model to simulate bacterial infectious diseases has shown to be a rapid, simple, and cost-effective alternative. The insect's innate immune response is remarkably similar to that of the vertebrates, and consists of both the cellular and the humoral immune response. Here, we provide a protocol for using G. mellonella larvae to study virulence of GAS, including the use of a health score system for quantitative analysis and the methods for assessing post-infection bacterial burden in vivo.
最近，利用大蜡螟幼虫作为非哺乳动物模型来模拟细菌感染性疾病已被证明是一种快速、简单且经济高效的替代方法。昆虫的先天免疫反应与脊椎动物的非常相似，包括细胞免疫反应和体液免疫反应。在此，我们提供了一种使用大蜡螟幼虫研究A群链球菌毒力的方案，包括使用健康评分系统进行定量分析以及评估感染后体内细菌负荷的方法。
