Laboratório de Bioquímica e Microbiologia, Departamento de Ciências Biológicas, Universidade do Estado de Minas Gerais, 36800-000, Carangola, MG, Brazil.
Laboratório de Microbiologia Molecular, Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, 21941-901, Rio de Janeiro, RJ, Brazil.
Pathog Dis. 2020 Nov 11;78(8). doi: 10.1093/femspd/ftaa056.
The larva of the greater wax moth Galleria mellonella is an increasingly popular model for assessing the virulence of bacterial pathogens and the effectiveness of antimicrobial agents. In this review, we discuss details of the components of the G. mellonella larval immune system that underpin its use as an alternative infection model, and provide an updated overview of the state of the art of research with G. mellonella infection models to study bacterial virulence, and in the evaluation of antimicrobial efficacy. Emphasis is given to virulence studies with relevant human and veterinary pathogens, especially Escherichia coli and bacteria of the ESKAPE group. In addition, we make practical recommendations for larval rearing and testing, and overcoming potential limitations of the use of the model, which facilitate intra- and interlaboratory reproducibility.
橘小实蝇幼虫是评估细菌病原体毒力和抗菌药物效果的一种越来越受欢迎的模型。在这篇综述中，我们讨论了支持其作为替代感染模型使用的橘小实蝇幼虫免疫系统成分的细节，并提供了最新的研究概述，使用橘小实蝇感染模型研究细菌毒力，并评估抗菌效果。重点是对相关的人类和兽医病原体，特别是大肠杆菌和 ESKAPE 组细菌的毒力研究。此外，我们还对幼虫饲养和测试提出了实用建议，并克服了该模型使用的潜在局限性，从而促进了实验室内部和实验室之间的可重复性。
