尿液pH值为1的提取物中的化合物与皮质类固醇和四氢皮质类固醇抗血清发生反应。分离出一种对四氢醛固酮抗血清有亲和力的非极性物质。

Compounds in the urinary pH 1 extract reacting with corticosteroid and tetrahydrocorticosteroid antisera. Separation of a nonpolar substance with affinity to a tetrahydroaldosterone antiserum.

作者信息

Lichtwald K, Connolly T M, Kohl K H, Vecsei P

出版信息

Klin Wochenschr. 1978;56 Suppl 1:169-72. doi: 10.1007/BF01477469.

Abstract

In human urinary pH 1 extracts prepared for the aldosterone-18-glucuronide estimation, several other substances are present, crossreacting not only with aldosterone antisera, but also with various corticosteroid and tetrahydrocorticosteroid antisera. Aldosterone was measured before and after chromatographic purification. Further characterization of the non-aldosterone immunoreactive material was made by immunological analysis of paper chromatogram eluats. Pregnancy, and administration of ACTH, dexamethasone, and metopirone led to a change of excretion in the antigenic equivalents. A method for the separation of the antigenic material is described. For structural elucidation the gaschromatography-mass spectrometry (GC-MS) method was applied.

摘要

在为醛固酮 - 18 - 葡萄糖醛酸酯测定而制备的人体尿液pH 1提取物中，存在几种其他物质，它们不仅与醛固酮抗血清发生交叉反应，还与各种皮质类固醇和四氢皮质类固醇抗血清发生交叉反应。在色谱纯化前后对醛固酮进行了测定。通过对纸色谱洗脱液的免疫分析对非醛固酮免疫反应性物质进行了进一步表征。妊娠以及促肾上腺皮质激素、地塞米松和甲吡酮的给药导致抗原当量排泄发生变化。描述了一种分离抗原物质的方法。为了进行结构解析，应用了气相色谱 - 质谱联用（GC - MS）方法。