Jiang Youde, Liu Li, Li Hainan, Wang Jie-Mei, Steinle Jena J
Department of Ophthalmology, Visual, and Anatomical Sciences, Wayne State University, Detroit, MI USA.
Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI USA.
J Diabetes Clin Res. 2020;2(1):12-15. Epub 2020 Mar 25.
Rates of type 2 diabetes are reaching epidemic levels. Yet, the tissue specific alterations due to insulin resistance are only recently being investigated. The goal of the present study was to evaluate retinal insulin signal transduction in a common mouse model of type 2 diabetes, the db/db mouse. Retinal lysates from five month old male db/db and db/+ (control) mice were collected and processed for Western blotting or ELISA analyses for insulin receptor, insulin receptor substrate-1 (IRS-1), Akt, tumor necrosis factor alpha (TNFα) and caspase 3 levels. Data demonstrate increased TNFα and IRS-1 phosphorylation on serine 307. This led to decreased Akt phosphorylation on serine 473 and increased cleavage of caspase 3. Taken together, the data suggest dysfunctional insulin signaling in the retina of the db/db mouse. insulin.
2型糖尿病的发病率正达到流行程度。然而，由于胰岛素抵抗导致的组织特异性改变直到最近才被研究。本研究的目的是评估2型糖尿病常见小鼠模型db/db小鼠的视网膜胰岛素信号转导。收集5个月大的雄性db/db和db/+（对照）小鼠的视网膜裂解物，并进行处理以用于胰岛素受体、胰岛素受体底物-1（IRS-1）、Akt、肿瘤坏死因子α（TNFα）和半胱天冬酶3水平的蛋白质印迹或酶联免疫吸附测定分析。数据显示TNFα和IRS-1在丝氨酸307处的磷酸化增加。这导致Akt在丝氨酸473处的磷酸化减少以及半胱天冬酶3的裂解增加。综上所述，数据表明db/db小鼠视网膜中的胰岛素信号传导功能失调。胰岛素。
