Department of Orthopedics, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, China.
Biomater Sci. 2020 Jun 21;8(12):3320-3333. doi: 10.1039/d0bm00467g. Epub 2020 May 20.
Magnesium hydroxide (Mg(OH)), magnesium fluoride (MgF), and hydroxyapatite (HA) films on Mg are widely studied owing to their easy preparation and favorable corrosion protection. Nevertheless, the most suitable film with the best performance for biomedical applications between the three films remains unknown. Therefore, the performance of the three coatings from in vitro to in vivo must be systematically investigated. In this study, Mg(OH), MgF, and HA films were fabricated on pure Mg. Electrochemical analysis and the hydrogen evolution test suggested that the HA film showed the best in vitro corrosion resistance, followed by MgF and Mg(OH) films. In vitro cell culture indicated that the extract of the MgF-coated sample was most beneficial for the osteogenic differentiation of MC3T3-E1 cells and the vascularization of human umbilical vein endothelial cells (HUVECs), which might be ascribed to the existence of the F element in the film. The result of this subcutaneous implantation showed that the HA film exhibited the best in vivo corrosion resistance and induced the lightest inflammatory response. Femoral implantation data revealed that the HA film exhibited the best osseointegration. Furthermore, the major organs and blood indicators of all of the tested rats were normal in 8 weeks. In summary, though the in vitro biological performance of the MgF film was the best among the three films, the HA film showed the best in vivo performance, suggesting that it is a more promising modification method for orthopedic applications.
氢氧化镁（Mg(OH)）、氟化镁（MgF）和羟基磷灰石（HA）薄膜由于其易于制备和良好的腐蚀防护性能而在 Mg 上得到了广泛的研究。然而，三种薄膜中哪一种最适合生物医学应用、性能最佳仍不得而知。因此，必须系统地研究这三种涂层从体外到体内的性能。在本研究中，在纯 Mg 上制备了 Mg(OH)、MgF 和 HA 薄膜。电化学分析和析氢试验表明，HA 薄膜在体外表现出最好的耐腐蚀性，其次是 MgF 和 Mg(OH)薄膜。体外细胞培养表明，MgF 涂层样品的浸提液最有利于 MC3T3-E1 细胞的成骨分化和人脐静脉内皮细胞（HUVEC）的血管生成，这可能归因于薄膜中存在 F 元素。皮下植入结果表明，HA 薄膜表现出最好的体内耐腐蚀性，并引起最轻的炎症反应。股骨植入数据显示，HA 薄膜表现出最好的骨整合。此外，所有测试大鼠的主要器官和血液指标在 8 周时均正常。综上所述，尽管 MgF 薄膜的体外生物性能在三种薄膜中最好，但 HA 薄膜在体内表现出最好的性能，表明其是一种更有前途的骨科应用改性方法。
