Sudor J, Stránský Z, Chmela Z, Bocek P
Department of Analytical Chemistry, Faculty of Natural Sciences, Palacky 1 University, Olomouc, Czechoslovakia.
Electrophoresis. 1988 Dec;9(12):799-803. doi: 10.1002/elps.1150091202.
The use of a bivalent counter anion for cationic isotachophoresis was studied both theoretically and experimentally. A mathematical model was proposed and solved to give the effective mobility of the hydrogen ion constituent, uH,H, in the respective electrolyte systems. The theoretical values agreed well with the experimental data in a series of electrolyte systems. The dependence of uH,H on such parameters as pH of the leading electrolyte, ionic mobilities of leading and counter ions, and dissociation constants was calculated and discussed. A bivalent counter anion will prove useful for the separation of low mobility cations and weak bases, as shown for the separation of substituted anilines in a succinate electrolyte system.
对用于阳离子等速电泳的二价抗衡阴离子进行了理论和实验研究。提出并求解了一个数学模型，以给出相应电解质体系中氢离子组分(u_{H,H})的有效迁移率。在一系列电解质体系中，理论值与实验数据吻合良好。计算并讨论了(u_{H,H})对前导电解质的pH值、前导离子和抗衡离子的离子迁移率以及解离常数等参数的依赖性。如在琥珀酸盐电解质体系中对取代苯胺的分离所示，二价抗衡阴离子将被证明对低迁移率阳离子和弱碱的分离有用。
