Ivanchenko M G, Georgieva E I
Institute of Genetics, Laboratory of Molecular Genetics, Bulgarian Academy of Sciences, Sofia.
Electrophoresis. 1988 Dec;9(12):812-5. doi: 10.1002/elps.1150091205.
Histones from maize embryos and seedlings have been isolated using a fast extraction procedure. Three different electrophoretic systems have been applied for the study of the heterogeneity of maize core histones. Electrophoresis in acetic acid/urea polyacrylamide gels, containing high concentrations of urea, resulted in optimum fractionation of the core histones and especially of histone H4. Sodium dodecyl sulfate-containing polyacrylamide gels were not useful for the fractionation of maize histone classes H2a and H2b, nor for the various subfractions of H3 and H4. Gels containing Triton X-100, used for the dimension in two-dimensional electrophoresis proved to be efficient for the separation of all histone classes, as well as their structural variants and chemical modifications. Maize core histones have been oxidized in an attempt to define which of the Triton X-100 resolved subfractions represent oxidation forms.
利用一种快速提取程序从玉米胚胎和幼苗中分离出了组蛋白。已应用三种不同的电泳系统来研究玉米核心组蛋白的异质性。在含有高浓度尿素的乙酸/尿素聚丙烯酰胺凝胶中进行电泳，可实现核心组蛋白尤其是组蛋白H4的最佳分级分离。含十二烷基硫酸钠的聚丙烯酰胺凝胶对于玉米组蛋白H2a和H2b的分级分离无用，对于H3和H4的各种亚组分也无用。用于二维电泳第二维的含Triton X-100凝胶被证明对于所有组蛋白类别及其结构变体和化学修饰的分离都是有效的。已对玉米核心组蛋白进行氧化，以确定Triton X-100分离出的哪些亚组分代表氧化形式。
