Berdnikov V A, Slobodianiuk S Ia
Biokhimiia. 1978 Jun;43(6):1001-5.
A simple method of microelectrophoresis for histone analysis from single fragments of polythenic chromosomes of chironomid salivary glands has been developed. The chromosomes or their fragments obtained micrurgically were dissolved in a microdroplet of 8 M urea and 1 N sulfuric acid solution. Electrophoresis was performed in 30--60 m diameter polyacrylamide gel cylinders under a layer of vaseline oil. The histones were separated into 6 electrophoretic bands which correspond to fractions H4, H2b, H2a+H3, H3-dimer, and two subfractions of histone H1. Comparison of histone electrophoregrams of the I, II, and III chromosomes showed their almost absolute identity. The ratio of fractions H4, H3, H2A, H2B as well as the ratio of histone H1 subfractions in the nucleus and in the rest of the IV chromosome cannot be visually distinguished.
已开发出一种用于分析摇蚊唾液腺多线染色体单个片段中组蛋白的简单微电泳方法。通过显微操作获得的染色体或其片段溶解在8M尿素和1N硫酸溶液的微滴中。电泳在凡士林油层下直径为30 - 60微米的聚丙烯酰胺凝胶柱中进行。组蛋白被分离成6条电泳带，分别对应于H4、H2b、H2a + H3、H3 - 二聚体以及组蛋白H1的两个亚组分。I、II和III号染色体的组蛋白电泳图谱比较显示它们几乎完全相同。在细胞核和IV号染色体其余部分中，H4、H3、H2A、H2B各组分的比例以及组蛋白H1亚组分的比例在视觉上无法区分。
