Advanced Water Management Centre, The University of Queensland, St. Lucia, Queensland 4072, Australia.
Environ Sci Technol. 2020 Jun 16;54(12):7629-7638. doi: 10.1021/acs.est.0c00912. Epub 2020 May 28.
Anaerobic technologies have been proposed as a promising solution to enhance bioenergy recovery and to transform a wastewater treatment plant (WWTP) from an energy consumer to an energy exporter. However, 20-60% of the methane produced remains dissolved in the anaerobically treated effluent, which is a potent greenhouse gas and is easily stripped out in the aeration tank. This study aims to develop a solution using dissolved methane to support denitrification, thus simultaneously enhancing nitrogen removal and achieving beneficial use of dissolved methane. By coupling anaerobic ammonium oxidation (anammox) with nitrite/nitrate-dependent anaerobic methane oxidation (n-DAMO), up to 85% of dissolved methane and more than 99% of nitrogen were removed in parallel in a biofilm system. Mass balance was conducted during both long-term operation and short-term batch tests, which indicated that n-DAMO bacteria and n-DAMO archaea indeed contributed jointly to the methane removal. The 16S rRNA gene amplicon sequencing further showed the co-presence of n-DAMO bacteria and n-DAMO archaea, while anammox bacteria were detected with a low relative abundance. This proposed technology can potentially be applied to reduce the carbon footprint and to save the organic carbon consumption in WWTPs.
厌氧技术被认为是一种很有前途的解决方案，可以提高生物能源的回收效率，并将污水处理厂（WWTP）从能源消费者转变为能源出口者。然而，20-60%的甲烷仍溶解在厌氧处理废水中，而这些甲烷是一种强大的温室气体，很容易在曝气池中被去除。本研究旨在开发一种利用溶解甲烷来支持反硝化的解决方案，从而同时提高氮的去除率，并实现溶解甲烷的有益利用。通过将厌氧氨氧化（anammox）与亚硝酸盐/硝酸盐依赖型厌氧甲烷氧化（n-DAMO）相结合，在生物膜系统中，高达 85%的溶解甲烷和超过 99%的氮可以同时去除。在长期运行和短期批量试验中进行了质量平衡，结果表明，n-DAMO 细菌和 n-DAMO 古菌确实共同促进了甲烷的去除。16S rRNA 基因扩增子测序进一步表明了 n-DAMO 细菌和 n-DAMO 古菌的共存，而 anammox 细菌的相对丰度较低。这项提出的技术可应用于减少 WWTP 的碳足迹并节省有机碳消耗。
