Günther S, Postel W, Wiering H, Görg A
Lehrstuhl für Allgemeine Lebensmitteltechnologie, Technische Universität München, Federal Republic of Germany.
Electrophoresis. 1988 Sep;9(9):618-20. doi: 10.1002/elps.1150090927.
The genetic variants of tomato acid phosphatase (Aps-1) systems have been analyzed by isoelectric focusing in immobilized pH gradients (IPG). By using an ultranarrow pH 4.25-4.55 IPG gel, the two genotypes Aps-1(1) and Aps-1+, differentiating tomato variants into nematode-resistant or nematode-susceptible plants, are separated into two sharp zones over a distance of 2.5 cm with isoelectric points of 4.37 and 4.43, respectively. Under these conditions, silver staining of the Aps-1 variants proved to be superior to enzyme staining. By applying more than 50 samples on one IPG gel, this method proved to be a powerful tool for reliable tomato nematode resistance screening.
通过在固定化pH梯度（IPG）中进行等电聚焦分析了番茄酸性磷酸酶（Aps-1）系统的遗传变异。使用超窄pH 4.25 - 4.55的IPG凝胶，将番茄变种分为抗线虫或感线虫植株的两种基因型Aps-1(1)和Aps-1+，在2.5厘米的距离内分别分离成两个清晰的区域，等电点分别为4.37和4.43。在这些条件下，Aps-1变体的银染被证明优于酶染。通过在一块IPG凝胶上应用50多个样品，该方法被证明是可靠的番茄线虫抗性筛选的有力工具。
