Burnley Gardens, Institute of Plant Sciences, Swan Street, 3121, Burnley, Victoria, Australia.
Theor Appl Genet. 1992 Nov;85(2-3):346-52. doi: 10.1007/BF00222880.
Barley yellow dwarf (BYDV) is a group a closely related viruses which cause economic losses in a wide range of graminaceous species throughout the world. Barley plants can be protected from the effects of BYDV by the 'Yd2' resistance gene. Plants which contain the 'Yd2' gene also contain a constitutively expressed polypeptide which was not found in any plants without 'Yd2'. Conversely, BYDV susceptible plants contain another constitutively expressed polypeptide which was not found in any of the BYDV-resistant lines examined. These two polypeptides appear to have the same molecular weight (as assessed by SDS-PAGE) and only slightly different iso-electric points. They also appear to contain an extensive range of similar antigenic determinants. Both polypeptides were found in F1 hybrids made from resistant and susceptible plants. We suggest that these two polypeptides are the products of two allelic genes. Analysis of near-isogenic lines showed that the locus which controls the 'Yd2' resistance gene and the locus controlling the synthesis of the two polypeptides may be within ± 9 cM of each other. We have developed a Western blot technique which allows assessment of barley lines, 4-days after seed imbibition, for the presence of the 'Yd2' gene.
大麦黄花叶病毒（BYDV）是一组密切相关的病毒，它们会在全世界范围内的多种禾本科植物中造成经济损失。大麦植物可以通过“Yd2”抗性基因来免受 BYDV 的影响。含有“Yd2”基因的植物还含有一种组成型表达的多肽，而在没有“Yd2”的植物中则没有发现这种多肽。相反，易感 BYDV 的植物含有另一种组成型表达的多肽，而在任何被检测的 BYDV 抗性系中都没有发现这种多肽。这两种多肽似乎具有相同的分子量（通过 SDS-PAGE 评估），只是等电点略有不同。它们似乎也含有广泛的类似抗原决定簇。这两种多肽都在来自抗性和易感植物的 F1 杂种中被发现。我们认为这两种多肽是两个等位基因的产物。对近等基因系的分析表明，控制“Yd2”抗性基因和两种多肽合成的基因座可能彼此相距±9 cM。我们已经开发出一种 Western blot 技术，可以在种子吸水后 4 天评估大麦品系中“Yd2”基因的存在。
