Guan A L, Markland F S
Department of Biochemistry, USC School of Medicine, Los Angeles 90033.
J Biochem Biophys Methods. 1988 Jun-Jul;16(2-3):215-26. doi: 10.1016/0165-022x(88)90032-2.
A fibrinolytic enzyme with a molecular weight between 23,000 and 25,000 Da has been purified from southern copperhead snake venom. Immobilized pH gradient isoelectric focusing with an ultranarrow pH interval (pH 6.65-6.95) resolved two isoforms of the fibrinolytic enzyme that were not resolved by standard isoelectric focusing. Attempts at purification of the individual isoenzymes by semi-preparative scale IPG and elution of enzyme by macerating the gel yielded only 20-40% recovery of activity. In attempts to improve recovery, a semi-preparative IPG canal-isoelectric focusing technique has been utilized.
