Tong L, Han W-Z, Wang J-L, Sun N-N, Zhuang M
Department of Respiratory Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, P.R. China.
Eur Rev Med Pharmacol Sci. 2020 May;24(9):4836-4845. doi: 10.26355/eurrev_202005_21172.
MicroRNAs (miRNAs) act as important regulators in human cancers by regulating the gene expression. The dysregulation of miR-365 has been investigated in many cancers. However, the function of miR-365 remains unknown in lung adenocarcinoma. Therefore, the regulatory mechanism of miR-365 was explored in lung adenocarcinoma.
The expression of miR-365 was detected in cell lines and 67 lung adenocarcinoma tissues using qRT-PCR. The Kaplan-Meier analysis was used to determine the association between miR-365 expressions and the survival rate in patients with lung adenocarcinoma. Transwell assay was then performed to investigate the effect of miR-365 on invasion and migration of lung adenocarcinoma cells.
Downregulation of miR-365 and upregulation of ETS1 were identified in lung adenocarcinoma. Furthermore, miR-365 reversely regulated ETS1 expression in lung adenocarcinoma. Functionally, the overexpression of miR-365 inhibited proliferation, migration, and invasion of lung adenocarcinoma cells. However, the upregulation of ETS1 lessened the inhibitory effect of miR-365 in lung adenocarcinoma. In addition, miR-365 inhibited EMT and inactivated AKT/mTOR pathway in lung adenocarcinoma.
MiR-365 inhibits the progression of lung adenocarcinoma by targeting ETS1 and inactivating the AKT/mTOR pathway.
微小RNA（miRNA）通过调控基因表达在人类癌症中发挥重要调节作用。miR-365的失调已在多种癌症中得到研究。然而，miR-365在肺腺癌中的功能仍不清楚。因此，本研究探讨了miR-365在肺腺癌中的调控机制。
采用qRT-PCR检测细胞系和67例肺腺癌组织中miR-365的表达。采用Kaplan-Meier分析确定miR-365表达与肺腺癌患者生存率之间的关系。然后进行Transwell实验以研究miR-365对肺腺癌细胞侵袭和迁移的影响。
在肺腺癌中发现miR-365表达下调，ETS1表达上调。此外，miR-365在肺腺癌中反向调控ETS1的表达。在功能上，miR-365的过表达抑制了肺腺癌细胞的增殖、迁移和侵袭。然而，ETS1的上调减弱了miR-365对肺腺癌的抑制作用。此外，miR-365抑制肺腺癌中的EMT并使AKT/mTOR通路失活。
MiR-365通过靶向ETS1并使AKT/mTOR通路失活来抑制肺腺癌的进展。
