Li Y, Zeng Q-G, Qiu J-L, Pang T, Wang H, Zhang X-X
Department of Thyroid and Breast Surgery, the Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, Guangdong, China.
Eur Rev Med Pharmacol Sci. 2020 May;24(9):5101-5110. doi: 10.26355/eurrev_202005_21203.
Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer and PTC patients with invasion and metastases features have a poor prognosis. Propofol is an intravenous anesthetic which has been reported to be involved in cancer progression. However, the roles of propofol and the exact molecular mechanisms in PTC remain largely unknown.
Cells viability was detected using the CCK-8 (Cell Counting Kit-8) assay. The expressions of microRNA-122 (miR-122) were measured by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Cells migration and invasion abilities were investigated by transwell. Western blot was used to demonstrate the expression of metastasis-and EMT-related proteins.
We found a significant inhibition of cells viability in TPC-1 and IHH-4 cells compared to Nthy-ori 3-1 cell line after exposed to propofol. The functional experiment showed propofol could suppress cells migration, invasion, and EMT in PTC. Subsequently, a decreased expression of miR-122 was detected in TPC-1 and IHH-4 cells, while a promotion of propofol on miR-122 expression was identified. Furthermore, we found miR-122 could inhibit cells migration, invasion, and EMT in PTC. Next, the rescue study indicated that miR-122 inhibitor transfection could attenuate propofol-induced suppression on TPC-1 and IHH-4 cells metastasis.
Propofol suppresses migration, invasion, and EMT in papillary thyroid carcinoma cells by regulating miR-122 expression. The findings may indicate significant clinical implications for anesthetic agents to prevent metastasis and improve outcomes in papillary thyroid carcinoma.
甲状腺乳头状癌（PTC）是最常见的甲状腺癌类型，具有侵袭和转移特征的PTC患者预后较差。丙泊酚是一种静脉麻醉剂，据报道其与癌症进展有关。然而，丙泊酚在PTC中的作用及确切分子机制仍 largely 未知。
使用CCK-8（细胞计数试剂盒-8）检测细胞活力。通过定量实时聚合酶链反应（qRT-PCR）测量微小RNA-122（miR-122）的表达。通过Transwell研究细胞迁移和侵袭能力。蛋白质印迹法用于证明转移和EMT相关蛋白的表达。
我们发现与Nthy-ori 3-1细胞系相比，TPC-1和IHH-4细胞在暴露于丙泊酚后细胞活力受到显著抑制。功能实验表明丙泊酚可抑制PTC中的细胞迁移、侵袭和EMT。随后，在TPC-1和IHH-4细胞中检测到miR-122表达降低，同时发现丙泊酚可促进miR-122表达。此外，我们发现miR-122可抑制PTC中的细胞迁移、侵袭和EMT。接下来，拯救研究表明miR-122抑制剂转染可减弱丙泊酚对TPC-1和IHH-4细胞转移的抑制作用。
丙泊酚通过调节miR-122表达抑制甲状腺乳头状癌细胞的迁移、侵袭和EMT。这些发现可能对麻醉剂预防甲状腺乳头状癌转移和改善预后具有重要临床意义。
