Department of Gynecology, Ningbo No. 6 Hospital, Ningbo, China.
Eur Rev Med Pharmacol Sci. 2020 May;24(9):4635-4641. doi: 10.26355/eurrev_202005_21148.
EMT is closely related to gene polymorphism and the expression level of immune-related substances in patients. Therefore, the aim of this study was to investigate the relationship between single nucleotide polymorphism (SNP), as well as serum levels of interleukin 2 (IL-2) and interleukin 6 (IL-6) in patients with endometriosis (EMT) and disease susceptibility.
Peripheral blood of EMT patients and healthy people were collected, respectively. Genomic deoxyribonucleic acid (DNA) was extracted and sequenced to obtain gene polymorphisms of IL-2 rs11575812 (T>C), rs2069772 (A>G), rs2069762 (T>G), and IL-6 rs1800795 (C>G). Meanwhile, the serum levels of IL-2 and IL-6 were determined by the relative kits.
For IL-2 rs11575812 allele (C>G), the odds ratio (OR) was 0.49, the 95% confidence interval (CI) was 0.37-0.66, and the p-value was 0. For IL-2 rs2069772 allele (C>G), the OR was 0.97, the 95% CI was 0.73-1.27, and the p-value was 0.83. For IL-2 rs2069762 allele (T>G), the OR was 1.73, the 95% CI was 1.31-2.29, and the p-value was 0. For IL-6 rs1800795 allele (C>G), the OR was 1.26, the 95% CI was 0.96-1.66, and the p-value was 0.09. CC genotype (p=0.000) and TT genotype (p=0.040) of IL-2 rs11575812 (T>C), AG genotype (p=0.000) of IL-2 rs2069772 (A>G), and GT genotype (p=0.000) of rs2069762 (T>G) were remarkably associated with the serum level of IL-2 in patients with EMT. Similarly, the CG genotype (p=0.000) of IL-6 rs1800795 (C>G) was significantly correlated with the serum level of IL-6 in patients with EMT. IL-2 haplotype CAG (p=0.005), CAT (p=0.001), CGG (p=0.047), TAG (p=0.000), and TGG (p=0.000) were significantly different from other haplotypes. Furthermore, there was a significant correlation between the serum levels of IL-2 and IL-6 (r=0.63, p<0.001).
IL-2 rs11575812 (T>C) TT genotype, rs2069772 (A>G) AG genotype and rs2069762 (T>G) GG genotype increases the risk of EMT, which are related to the serum levels of IL-2 and IL-6.
EMT 与患者基因多态性和免疫相关物质的表达水平密切相关。因此，本研究旨在探讨子宫内膜异位症（EMT）患者单核苷酸多态性（SNP）以及白细胞介素 2（IL-2）和白细胞介素 6（IL-6）血清水平与疾病易感性的关系。
分别采集 EMT 患者和健康人的外周血，提取基因组脱氧核糖核酸（DNA）并进行测序，以获得 IL-2 rs11575812（T>C）、rs2069772（A>G）、rs2069762（T>G）和 IL-6 rs1800795（C>G）的基因多态性。同时，采用相对试剂盒测定 IL-2 和 IL-6 的血清水平。
IL-2 rs11575812 等位基因（C>G）的优势比（OR）为 0.49，95%置信区间（CI）为 0.37-0.66，p 值为 0。IL-2 rs2069772 等位基因（C>G）的 OR 为 0.97，95%CI 为 0.73-1.27，p 值为 0.83。IL-2 rs2069762 等位基因（T>G）的 OR 为 1.73，95%CI 为 1.31-2.29，p 值为 0。IL-6 rs1800795 等位基因（C>G）的 OR 为 1.26，95%CI 为 0.96-1.66，p 值为 0.09。IL-2 rs11575812（T>C）的 CC 基因型（p=0.000）和 TT 基因型（p=0.040）、IL-2 rs2069772（A>G）的 AG 基因型（p=0.000）和 rs2069762（T>G）的 GT 基因型与 EMT 患者 IL-2 的血清水平显著相关。同样，IL-6 rs1800795（C>G）的 CG 基因型（p=0.000）与 EMT 患者 IL-6 的血清水平显著相关。IL-2 单倍型 CAG（p=0.005）、CAT（p=0.001）、CGG（p=0.047）、TAG（p=0.000）和 TGG（p=0.000）与其他单倍型明显不同。此外，IL-2 和 IL-6 的血清水平之间存在显著相关性（r=0.63，p<0.001）。
IL-2 rs11575812（T>C）TT 基因型、rs2069772（A>G）AG 基因型和 rs2069762（T>G）GG 基因型增加 EMT 的发病风险，与 IL-2 和 IL-6 的血清水平有关。
