Wójtowicz Mariusz, Zdun Dariusz, Owczarek Aleksander Jerzy, Skrzypulec-Plinta Violetta, Olszanecka-Glinianowicz Magdalena
Clinical Department of Gynecology and Obstetrics, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland.
Health Promotion and Obesity Management Unit, Department of Pathophysiology, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, 40-055 Katowice, Poland.
Int J Mol Sci. 2025 May 26;26(11):5117. doi: 10.3390/ijms26115117.
Some studies indicate the role of TNF-α, IL-6, and IL-8 in the development of endometriosis. However, a comprehensive assessment of plasma, peritoneal, and endometrioma fluids of proinflammatory cytokine concentrations in women with ovarian endometriosis has not yet been performed. Therefore, this study aimed to analyze plasma, peritoneal, and endometrioma fluids for selected proinflammatory cytokine concentrations in women operated on for ovarian endometriosis. A retrospective study was conducted with 56 women who underwent surgery for ovarian endometriosis. Body mass, height, and waist circumference were measured, as well as BMI being calculated. Plasma, peritoneal, and endometrioma fluids' interleukin-6 (IL-6), IL-8, and tumor necrosis factor-alpha (TNF-α) were determined by ELISA. Levels of IL-6 and TNF-α were significantly higher in endometrioma fluid compared to plasma and peritoneal fluid. In addition, levels of IL-6 and TNF-α were significantly higher in peritoneal fluid than found in plasma. Levels of IL-8 did not significantly differ between plasma and both peritoneal and endometrioma fluids, or between peritoneal and endometrioma fluids. There were also positive correlations among IL-6, IL-8, and TNF-α levels in endometrioma and peritoneal fluids (ρ = 0.29; < 0.05; ρ = 0.51; < 0.001; ρ = 0.52; < 0.001, respectively). There were no associations between cytokine levels in plasma, peritoneal, and endometrioma fluids and endometriosis stage. Plasma IL-8 levels can be considered an emerging biomarker of severity of local inflammation related to endometrioma. Further studies are needed for understanding the role of IL-6 and TNF-α as the markers of local inflammation in endometrioma.
一些研究表明肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）在子宫内膜异位症的发展中起作用。然而，尚未对卵巢子宫内膜异位症女性的血浆、腹腔液和子宫内膜瘤液中的促炎细胞因子浓度进行全面评估。因此，本研究旨在分析接受卵巢子宫内膜异位症手术的女性的血浆、腹腔液和子宫内膜瘤液中选定促炎细胞因子的浓度。对56例接受卵巢子宫内膜异位症手术的女性进行了一项回顾性研究。测量了体重、身高和腰围，并计算了体重指数（BMI）。通过酶联免疫吸附测定法（ELISA）测定血浆、腹腔液和子宫内膜瘤液中的白细胞介素-6（IL-6）、IL-8和肿瘤坏死因子-α（TNF-α）。与血浆和腹腔液相比，子宫内膜瘤液中IL-6和TNF-α的水平显著更高。此外，腹腔液中IL-6和TNF-α的水平显著高于血浆中的水平。血浆与腹腔液和子宫内膜瘤液之间，以及腹腔液与子宫内膜瘤液之间的IL-8水平没有显著差异。子宫内膜瘤液和腹腔液中IL-6、IL-8和TNF-α水平之间也存在正相关（相关系数分别为ρ = 0.29；P < 0.05；ρ = 0.51；P < 0.001；ρ = 0.52；P < 0.001）。血浆、腹腔液和子宫内膜瘤液中的细胞因子水平与子宫内膜异位症分期之间没有关联。血浆IL-8水平可被视为与子宫内膜瘤相关的局部炎症严重程度的新兴生物标志物。需要进一步研究以了解IL-6和TNF-α作为子宫内膜瘤局部炎症标志物的作用。
