进展期和无复发多发性硬化症患者血清 miR-128-3p 上调。

Upregulated serum miR-128-3p in progressive and relapse-free multiple sclerosis patients.

机构信息

Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona - Neurology Section, Verona, Italy.

Department of Neurosciences, Biomedicine and Movement Sciences, University of Verona - Biology and Genetics Section, Verona, Italy.

出版信息

Acta Neurol Scand. 2020 Nov;142(5):511-516. doi: 10.1111/ane.13288. Epub 2020 Jun 5.

DOI:10.1111/ane.13288

PMID:32432792

Abstract

BACKGROUND

Circulating microRNAs have emerged as novel multiple sclerosis (MS) biomarkers.

AIMS

To assess the association between candidate miR expression in serum samples of patients with MS and the disease course.

METHODS

Serum levels of ten microRNAs (ie, miR-199, miR-128-3p, miR-20a-5p, miR-27a-3p, miR-15b-5p, miR-325, miR-92a1-5p, miR-223-5p, miR-22-5p, and miR-23a-5p) were measured in 74 MS cases and 17 non-MS controls consecutively enrolled at Verona University Hospital. The association of microRNA expression with patients' clinical and MRI features was analyzed. Candidate microRNAs were detected by real-time PCR and expressed as ratio of each microRNA level to a normalizer.

RESULTS

Serum miR-128-3p levels were higher in progressive than relapsing MS (median ratio 2.86 vs 0.73, P = .036). In addition, miR-128-3p was upregulated in patients without relapses after sample collection compared to cases who relapsed (1.64 vs 0.82; P = .014). miR-128-3p levels and relapse rate were inversely correlated (r = -.44, P = .008).

CONCLUSIONS

Serum levels of mir-128-3p could be related to biological mechanisms underlying MS activity and progression.

摘要

背景

循环 microRNAs 已成为新型多发性硬化症（MS）生物标志物。

目的

评估 MS 患者血清样本中候选 miR 表达与疾病病程之间的相关性。

方法

连续纳入维罗纳大学医院的 74 例 MS 病例和 17 例非 MS 对照者，检测血清中 10 种 microRNAs（miR-199、miR-128-3p、miR-20a-5p、miR-27a-3p、miR-15b-5p、miR-325、miR-92a1-5p、miR-223-5p、miR-22-5p 和 miR-23a-5p）的水平。分析 microRNA 表达与患者临床和 MRI 特征的关系。采用实时 PCR 检测候选 microRNAs，并用每个 microRNA 水平与归一化因子的比值表示。

结果

进展性 MS 患者血清 miR-128-3p 水平高于复发性 MS（中位数比值 2.86 比 0.73，P =.036）。此外，与复发患者相比，在采集样本后未复发的患者中，miR-128-3p 上调（1.64 比 0.82；P =.014）。miR-128-3p 水平与复发率呈负相关（r = -.44，P =.008）。