Department of Physical Chemistry, School of Pharmaceutical Sciences, Jinzhou Medical University, Jinzhou, Liaoning, People's Republic of China.
IET Nanobiotechnol. 2020 Apr;14(2):167-171. doi: 10.1049/iet-nbt.2019.0257.
The electrochemiluminescence (ECL) system based on the ruthenium complex has become a powerful tool in the field of analytical chemistry. However, the non-aqueous ECL luminescence system, which does not involve complex nano-modification, has not been widely used for the determination of analytes. In this study, -methyl pyrrolidone was selected as the solvent, and it could also act as a co-reactant of [inline-formula removed]. Based on this, a simple ECL system without nanomaterials was established. Strong ECL was generated. Furthermore, a quenching effect between the excited state of [inline-formula removed] and sulphamethoxazole (SMZ) was observed. Based on this, a sensitive ECL sensor for detecting SMZ is constructed. A linear relationship between ECL signal quenching intensity (Δ) and the logarithm of SMZ concentration (log ) in the concentration range of 1 × 10-1 × 10 mol/l is obtained. The limit of detection is as low as 3.33 × 10 mol/l. The method has been applied to the detection of SMZ in tap water samples with different concentration levels with satisfactory results, and the recovery was 95.3-102.6%.
基于钌配合物的电化学发光（ECL）体系已成为分析化学领域的有力工具。然而，不涉及复杂纳米修饰的非水相 ECL 发光体系尚未广泛用于分析物的测定。在本研究中，选择 N-甲基吡咯烷酮作为溶剂，同时其也可作为[inline-formula removed]的共反应物。基于此，建立了一种无需纳米材料的简单 ECL 体系，可产生强 ECL。此外，观察到[inline-formula removed]的激发态与磺胺甲恶唑（SMZ）之间存在猝灭效应。基于此，构建了一种用于检测 SMZ 的灵敏 ECL 传感器。在 1×10-1×10 mol/L 的浓度范围内，ECL 信号猝灭强度（Δ）与 SMZ 浓度对数（log ）之间存在线性关系。检测限低至 3.33×10 mol/L。该方法已成功应用于不同浓度水平自来水中 SMZ 的检测，回收率为 95.3-102.6%。
