Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian Molecular Medicine, Key Laboratory of Xiamen Marine and Gene Drugs, Key Laboratory of Precision Medicine and Molecular Diagnosis of Fujian Universities, School of Biomedical Sciences, Huaqiao University, Xiamen, 361021, PR China.
Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian Molecular Medicine, Key Laboratory of Xiamen Marine and Gene Drugs, Key Laboratory of Precision Medicine and Molecular Diagnosis of Fujian Universities, School of Biomedical Sciences, Huaqiao University, Xiamen, 361021, PR China.
Chemosphere. 2020 Oct;256:127037. doi: 10.1016/j.chemosphere.2020.127037. Epub 2020 May 13.
Succinate dehydrogenase inhibitor (SDHI) fungicides are extensively used in agriculture. Some SDHI fungicides show developmental toxicity, immune toxicity and hepatotoxicity to fish. Fluxapyroxad (FLU) is a broad spectrum pyrazole-carboxamide SDHI fungicide and its potential impacts on fish embryonic development are unknown. We exposed zebrafish embryos to 1, 2 and 4 μM FLU. Developmental malformations, including yolk sac absorption disorder, decreased pigmentation and hatch delay were induced after FLU exposure. FLU caused significantly increased transcription levels in the ectoderm marker foxb1a but no significant changes in endoderm and mesoderm development markers (foxa2, ntl and eve1). Transcription levels of genes in the early stage embryos (gh, crx, neuroD and nkx2.4b) decreased significantly after FLU treatments. The content of glutathione (GSH) increased after FLU exposure. This study shows that FLU is toxic to zebrafish through its developmental effects and oxidative stress. FLU may pose risks to other non-target aquatic organisms.
琥珀酸脱氢酶抑制剂 (SDHI) 杀菌剂在农业中被广泛使用。一些 SDHI 杀菌剂对鱼类具有发育毒性、免疫毒性和肝毒性。氟唑菌酰胺 (FLU) 是一种广谱吡唑羧酰胺 SDHI 杀菌剂，其对鱼类胚胎发育的潜在影响尚不清楚。我们将斑马鱼胚胎暴露于 1、2 和 4 μM 的 FLU 中。FLU 暴露后，会诱导出卵黄囊吸收障碍、色素沉着减少和孵化延迟等发育畸形。FLU 导致外胚层标记物 foxb1a 的转录水平显著增加，但对内胚层和中胚层发育标记物（foxa2、ntl 和 eve1）没有显著变化。FLU 处理后，早期胚胎（gh、crx、neuroD 和 nkx2.4b）的基因转录水平显著降低。暴露于 FLU 后，谷胱甘肽 (GSH) 的含量增加。这项研究表明，FLU 通过其发育效应和氧化应激对斑马鱼产生毒性。FLU 可能对其他非靶标水生生物构成风险。
