采用超高效液相色谱/串联质谱法同时测定水果中的替芬那酯及其 6 种代谢物。
Simultaneous determination of tiafenacil and its six metabolites in fruits using ultra-high-performance liquid chromatography/tandem mass spectrometry.
机构信息
Guangxi Key Laboratory of Agric-Environment and Agric-products Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi 530004, People's Republic of China.
Guangxi Key Laboratory of Agric-Environment and Agric-products Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi 530004, People's Republic of China.
出版信息
Food Chem. 2020 Oct 15;327:127015. doi: 10.1016/j.foodchem.2020.127015. Epub 2020 May 11.
A simple and reliable analytical method was established for the simultaneous determination of tiafenacil and its six metabolites in fruits. The method involves three steps: extraction using acidified acetonitrile, clean-up by octadecylsilane(C18) and graphitized carbon black (GCB), and detection using liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The method was validated on seven matrices spiked at 10, 100 and 1000 μg kg. Average recoveries ranged from 73% to 105% with intra-day RSDr (n = 5) of 1.0%-13.0% and inter-day RSD (n = 15) of 1.1%-14.6%. Good linearities (R > 0.9911) were obtained for seven analytes in all matrices. The limit of quantification (LOQs) for tiafenacil and its six metabolites were 10 μg kg in all matrices. This analytical method provides a basis for the establishment of maximum residue limits (MRL) and for the monitoring of tiafenacil residues in fruits.
建立了一种简单可靠的分析方法，用于同时测定水果中的噻吩菌酯及其六种代谢物。该方法包括三个步骤：酸化乙腈提取、十八烷基硅烷（C18）和石墨化碳黑（GCB）净化以及液相色谱-串联质谱（UHPLC-MS/MS）检测。该方法在 10、100 和 1000μg/kg 三个添加水平下对七种基质进行了验证。平均回收率在 73%至 105%之间，日内 RSDr（n=5）为 1.0%-13.0%，日间 RSD（n=15）为 1.1%-14.6%。在所有基质中，七种分析物均具有良好的线性（R>0.9911）。噻吩菌酯及其六种代谢物在所有基质中的定量限（LOQs）均为 10μg/kg。该分析方法为水果中噻吩菌酯残留的最大残留限量（MRL）的制定和监测提供了依据。
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