Schusterova Dana, Hajslova Jana, Kocourek Vladimir, Pulkrabova Jana
Department of Food Analysis and Nutrition, University of Chemistry and Technology Prague, Technicka 3, 166 28 Prague, Czech Republic.
Foods. 2021 Feb 2;10(2):307. doi: 10.3390/foods10020307.
In this study, the occurrence of pesticide residues and their metabolites in grapes and wines was investigated. A targeted analysis of 406 pesticide residues in 49 wine and grape samples from organic and conventional production were performed using the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction method, followed by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry. Multiple residues (>4 residues/sample) were detected in 22 tested samples. The most commonly detected residues were fungicides (e.g., boscalid) and insecticides (e.g., methoxyfenozide). An ultra-high-performance liquid chromatography-high resolution mass spectrometry method (UHPLC-(HR)MS) was used for screening of pesticide metabolites. We also provide a method and database for detecting pesticide metabolites (extending our previously published database to 49 metabolites originating from 25 pesticides). An introduced strategy of targeted screening of pesticide metabolites was applied for authentication of 27 organic grapes and wines. In total, 23 samples were free of quantifiable residues/detected metabolites or contained residues approved for organic production.
在本研究中，对葡萄和葡萄酒中农药残留及其代谢物的存在情况进行了调查。使用QuEChERS（快速、简便、廉价、有效、耐用且安全）提取方法，对49个来自有机和常规生产的葡萄酒及葡萄样品中的406种农药残留进行了靶向分析，随后采用超高效液相色谱-串联质谱法。在22个测试样品中检测到多种残留（>4种残留/样品）。最常检测到的残留为杀菌剂（如啶酰菌胺）和杀虫剂（如甲氧虫酰肼）。采用超高效液相色谱-高分辨率质谱法（UHPLC-(HR)MS）筛查农药代谢物。我们还提供了一种检测农药代谢物的方法和数据库（将我们之前发布的数据库扩展至源自25种农药的49种代谢物）。一种引入的靶向筛查农药代谢物的策略被应用于27种有机葡萄和葡萄酒的认证。总共23个样品没有可量化的残留/检测到的代谢物，或者含有有机生产中批准使用的残留。
