Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
Laboratory of Theriogenology, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
J Vet Med Sci. 2020 Jul 10;82(7):926-934. doi: 10.1292/jvms.20-0165. Epub 2020 May 20.
This study aimed to describe the duration of inflammation after intrauterine infusion of polyvinylpyrrolidone-iodine (povidone-iodine, PVP-I), determine the effect of PVP-I infusion on the subsequent fertility, and evaluate the histopathology of the endometrium in dairy cows. In Experiment 1, 120 lactating clinically healthy Holstein-Friesian cows at 5 weeks postpartum (W5) were equally divided into three groups: intrauterine infusion of 2% PVP-I (PVP), saline (SAL), and no treatment (NTX). Endometrial cytology was performed daily from D0 (W5) to D7 to determine the percentage of polymorphonuclear cells (PMN%) in 44 of the 120 cows. All cows received timed artificial insemination at D17. In Experiment 2, 25 cows were randomly classified into sacrifice at 24 hr or 48 hr after 2% PVP-I infusion (PVP24 and PVP48), and 24, 48, 72, or 96 hr after SAL infusion (SAL24; SAL48; SAL72; SAL96), or no treatment (NTX). Histopathology was performed on the uterus of each cow. In Experiment 1, PMN% was greater in PVP (P<0.05) than in SAL and NTX, on D1, but decreased to a level similar to that of the other groups by D2. Conception rate was higher (P<0.05) in PVP cows compared to SAL and NTX cows. In Experiment 2, stratified columnar epithelium in the uterus disappeared in PVP24 and SAL24. The epithelium was regenerated in PVP48, SAL72, and SAL96, but not in SAL48. In conclusion, the results of the study suggest that PVP-I induces transient uterine inflammation, promotes regeneration of endometrial epithelial cells and improves fertility.
本研究旨在描述聚维酮碘（PVP-I）宫腔内灌注后炎症的持续时间，确定 PVP-I 灌注对随后的生育力的影响，并评估奶牛子宫内膜的组织病理学变化。在实验 1 中，将 120 头产后 5 周（W5）的临床健康荷斯坦弗里森奶牛等分为三组：2%PVP-I（PVP）宫腔内灌注组、生理盐水（SAL）灌注组和无处理组（NTX）。从 D0（W5）到 D7，对 120 头奶牛中的 44 头进行子宫内膜细胞学检查，以确定多形核细胞（PMN%）的百分比。所有奶牛均在 D17 进行定时人工授精。在实验 2 中，25 头奶牛随机分为 2%PVP-I 灌注后 24 小时（PVP24）和 48 小时（PVP48）处死组，以及 SAL 灌注后 24 小时（SAL24）、48 小时（SAL48）、72 小时（SAL72）和 96 小时（SAL96）处死组和无处理组（NTX）。对每头奶牛的子宫进行组织病理学检查。在实验 1 中，PVP 组奶牛的 PMN%在 D1 时高于 SAL 组和 NTX 组（P<0.05），但在 D2 时降至与其他组相似的水平。与 SAL 组和 NTX 组相比，PVP 组奶牛的受胎率更高（P<0.05）。在实验 2 中，子宫的柱状上皮在 PVP24 和 SAL24 组中消失。PVP48、SAL72 和 SAL96 组的上皮再生，但 SAL48 组没有。综上所述，该研究结果表明，PVP-I 可引起短暂的子宫炎症，促进子宫内膜上皮细胞的再生，提高生育力。
