Khuwaja Gulrana, Al-Bratty Mohammed, Alhazmi Hassan A, Khan Andleeb, Safhi Mohammad M, Ashafaq Mohammad, Islam Farha, Islam Fakhrul, Taha Manal M
Department of Pharmaceutical Chemistry, College of Pharmacy, Jazan University, Jazan, Saudi Arabia.
Substance Abuse Research Centre (SARC), College of Pharmacy, Jazan University, Jazan, Saudi Arabia.
Saudi Pharm J. 2020 May;28(5):630-636. doi: 10.1016/j.jsps.2020.04.002. Epub 2020 Apr 10.
The present research was designed to evaluate the toxicity of tellurium and its prevention by selenium on the pituitary gland in male Wistar rats.
30 rats were used weighing 200-250 gm, and randomly divided them into five groups. Each group contained an equal number of animals. Group-1 was nominated as control group. Group-2 received an intraperitoneal dose of selenium 0.3 mg per kg body wt. Group-3 was administered with tellurium 4.15 mg per kg body wt. Group-4 was given low-dose (L) of both selenium 0.15 and tellurium 2.075, Group-5 was given High-dose (H) of both selenium 0.3 and tellurium 4.15 mg/kg body wt. orally once in a day. After 15 days of dosing, the behavioral activities- motor co-ordination rotarod and grip strength test were measured. On 16th-day animals were sacrificed and activity of LPO, GSH, caspase-3, caspase-9, GPx, GR, SOD, catalase, and AChE were performed on the pituitary gland as per standard method reported.
Se when given together with Te, significantly protects the motor coordination up to 32.5%, and also protects the grip strength up to 75% in group 4 and 5 respectively as compared to group- 3. Se + Te treatment protects the activity of TBARS up to 48.68% and GSH is 58%. As compared to control, it protects caspase-3 up to 118% and caspase-9 up to 83%. The level of AChE was also observed to be modulated by the administration of Se in Group- 4 and 5. Se + Te protected AChE up to 28.6%. Similar findings were observed for the biochemical activities of GPx (140% protection), SOD (458%), GR (159%), and catalase (95%) activities that were protected significantly Se + Te in Group- 4 and 5.
Selenium dose-dependently protects behavioral activities. It also protects apoptosis, oxidative stress, and AChE activities in the pituitary gland.
本研究旨在评估碲的毒性以及硒对雄性Wistar大鼠垂体的预防作用。
选用30只体重200 - 250克的大鼠，随机分为五组。每组动物数量相等。第1组被指定为对照组。第2组腹腔注射每千克体重0.3毫克的硒。第3组给予每千克体重4.15毫克的碲。第4组给予低剂量（L）的硒0.15毫克和碲2.075毫克，第5组给予高剂量（H）的硒0.3毫克和碲4.15毫克/千克体重，每日口服一次。给药15天后，测量行为活动——运动协调性转棒试验和握力测试。在第16天处死动物，并按照报道的标准方法对垂体进行脂质过氧化（LPO）、谷胱甘肽（GSH）、半胱天冬酶 - 3（caspase - 3）、半胱天冬酶 - 9（caspase - 9）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GR）、超氧化物歧化酶（SOD）、过氧化氢酶（catalase）和乙酰胆碱酯酶（AChE）活性检测。
与第3组相比，硒与碲联合给药时，第4组和第5组分别显著保护了高达32.5%的运动协调性和高达75%的握力。硒 + 碲处理使丙二醛（TBARS）活性保护高达48.68%，谷胱甘肽（GSH）活性保护高达58%。与对照组相比，它使半胱天冬酶 - 3保护高达118%，半胱天冬酶 - 9保护高达83%。在第4组和第5组中，还观察到乙酰胆碱酯酶（AChE）水平受硒给药的调节。硒 + 碲使乙酰胆碱酯酶保护高达28.6%。在第4组和第5组中，对于谷胱甘肽过氧化物酶（GPx）（保护140%）、超氧化物歧化酶（SOD）（保护458%）、谷胱甘肽还原酶（GR）（保护159%）和过氧化氢酶（catalase）（保护95%）的生化活性也观察到类似的显著保护结果。
硒剂量依赖性地保护行为活动。它还保护垂体中的细胞凋亡、氧化应激和乙酰胆碱酯酶活性。
