通过原位杂交和缺失图谱分析将葡萄糖-6-磷酸脱氢酶（G6PD）和次黄嘌呤磷酸核糖基转移酶（HPRT）定位到北美有袋动物（弗吉尼亚负鼠）X染色体的不同臂上：进化意义

Localization of G6PD and HPRT to different arms of the X chromosome of the North American marsupial (Didelphis virginiana) by in situ hybridization and deletion mapping: evolutionary significance.

作者信息

Driscoll D J, Migeon B R

机构信息

Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

出版信息

Genomics. 1988 Nov;3(4):308-14. doi: 10.1016/0888-7543(88)90121-8.

DOI:10.1016/0888-7543(88)90121-8

PMID:3243545

Abstract

We have mapped HPRT and G6PD loci on the X chromosome in the American opossum, Didelphis virginiana, by in situ hybridization to cells derived from two females by using genomic opossum DNA as probes. The localizations (G6PD to Xp13 and HPRT to Xq21), indicating that the two genes are separated by the centromere, were confirmed by results of hybridization to X chromosomes with deletions that include the HPRT locus and opossum-mouse cell hybrids containing the relevant fragment of the opossum X chromosome.

摘要

我们通过使用负鼠基因组DNA作为探针，对源自两只雌性负鼠的细胞进行原位杂交，将次黄嘌呤磷酸核糖转移酶（HPRT）和葡萄糖-6-磷酸脱氢酶（G6PD）基因座定位到了北美负鼠（Didelphis virginiana）的X染色体上。通过与包含HPRT基因座的缺失X染色体以及含有负鼠X染色体相关片段的负鼠-小鼠细胞杂种进行杂交，证实了基因定位结果（G6PD位于Xp13，HPRT位于Xq21），这表明这两个基因被着丝粒隔开。