Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, V.le F. Stagno D'Alcontres 31, 98166, Messina, Italy.
Department of Veterinary Sciences, Zebrafish Neuromorphology Lab, University of Messina, Polo Universitario Dell'Annunziata, 98168, Messina, Italy.
Histochem Cell Biol. 2020 Nov;154(5):495-505. doi: 10.1007/s00418-020-01883-9. Epub 2020 May 20.
In the last decade, zebrafish has been used as a model for the study of several human skin diseases. The epidermis of Danio rerio is composed of keratinocytes and two types of secretory cells: mucous cells and club cells. Club cells have multiple biological functions and among them may be important in the protection against ultraviolet damage through the proliferative response or through the increased production of protective substances. Calcium-binding proteins such as calbindin D28K and calretinin are used as markers of nervous and enteric nervous systems, but they are present in numerous other cells. These proteins are involved in a wide variety of cell activities, such as cytoskeletal organization, cell motility and differentiation, cell cycle regulation and neuroprotective function. In this study we demonstrated, for the first time, the presence of calretinin and calbindin D28K in skin club cells of Danio rerio exposed to different wavelengths by immunohistochemistry analysis. Exposure to white-blue light and blue light causes the expression and colocalization of calbindin-D28K and calretinin. These proteins were moderately expressed and no colocalization was observed in the club cells of the control fish. In zebrafish exposed to continuous darkness for 10 days, in the club cells the two antibodies did not detect any proteins specifically. These results demonstrate that calbindin and calretinin could be involved in the pathophysiology of skin injury due to exposure to short-wavelength visible light spectrums.
在过去的十年中，斑马鱼已被用作研究几种人类皮肤疾病的模型。Danio rerio 的表皮由角质形成细胞和两种分泌细胞组成：粘液细胞和棒状细胞。棒状细胞具有多种生物学功能，其中之一可能是通过增殖反应或通过增加保护性物质的产生来保护免受紫外线损伤。钙结合蛋白，如钙结合蛋白 D28K 和钙视网膜蛋白，被用作神经和肠神经系统的标志物，但它们也存在于许多其他细胞中。这些蛋白质参与各种细胞活动，如细胞骨架组织、细胞运动和分化、细胞周期调节和神经保护功能。在这项研究中，我们首次通过免疫组织化学分析证实了暴露于不同波长的斑马鱼皮肤棒状细胞中存在钙视网膜蛋白和钙结合蛋白 D28K。暴露于白-蓝光和蓝光会导致钙结合蛋白-D28K 和钙视网膜蛋白的表达和共定位。这些蛋白质在对照组鱼的棒状细胞中表达适度，没有观察到共定位。在连续黑暗中暴露 10 天的斑马鱼中，两种抗体在棒状细胞中均未检测到任何特定的蛋白质。这些结果表明，钙结合蛋白和钙视网膜蛋白可能参与由于暴露于短波长可见光光谱而导致的皮肤损伤的病理生理学。
