Department of Clinical Immunology, Laboratory of Immunogenetics and Tissue Immunology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
Department of Reproductive Medicine, Gameta Hospital, Rzgów, Poland.
J Assist Reprod Genet. 2020 Aug;37(8):2007-2017. doi: 10.1007/s10815-020-01814-6. Epub 2020 May 20.
Approximately 50% of men reporting to clinics for assisted reproduction have abnormal sperm parameters; we therefore considered whether they differ from fertile males in terms of the frequency of KIR and HLA-C genes, suggesting the involvement of NK cells and some T cells in the inflammatory reaction that can occur in the testes, vas deferens, or epididymis.
We tested a total of 1064 men: 445 of them were patients who, together with their female partners, participated in in vitro fertilization (IVF), 298 men whose female partners suffered from recurrent spontaneous abortion. Three hundred twenty-one fertile men constituted the control group. KIRs were genotyped using KIR Ready Gene kits and HLA-C by PCR-SSP methods.
We found differences in KIR gene frequencies between men who became fathers via natural conception and men who participated in in vitro fertilization for KIR2DL2 (p/p = 0.0015/0.035, OR = 1.61), KIR2DL5 gr.2 (p/p = 0.0023/0.05, OR = 1.64), KIR2DS2 (p/p = 0.0019/0.044, OR = 1.59), and KIR2DS3 (p/p = 0.0016/0.037, OR = 1.67). KIRs in Cen AA region were significantly overrepresented in fertile males than in IVF males (p/p = 0.0076/0.03, OR = 0.67), whereas Cen AB + Cen BB frequency was higher in IVF males than in fertile males (p/p = 0.0076/0.03, OR = 1.50). We also observed a limited association in KIR-HLA-C combinations.
Fertile men differ in profile of KIR genes and KIR-HLA-C combinations from men participating in IVF.
约 50%前往诊所进行辅助生殖的男性存在精子参数异常；因此，我们考虑了他们的杀伤细胞免疫球蛋白样受体（KIR）和人类白细胞抗原-C（HLA-C）基因频率是否与生育男性不同，这提示自然杀伤（NK）细胞和某些 T 细胞可能参与睾丸、输精管或附睾中发生的炎症反应。
我们共检测了 1064 名男性：其中 445 名是与伴侣一起接受体外受精（IVF）的患者，298 名是伴侣反复自然流产的男性，321 名是生育能力正常的男性。我们使用 KIR Ready Gene 试剂盒检测 KIR 基因型，使用 PCR-SSP 方法检测 HLA-C。
我们发现自然受孕生育的男性与接受 IVF 的男性之间 KIR 基因频率存在差异，KIR2DL2（p/p=0.0015/0.035，OR=1.61）、KIR2DL5gr.2（p/p=0.0023/0.05，OR=1.64）、KIR2DS2（p/p=0.0019/0.044，OR=1.59）和 KIR2DS3（p/p=0.0016/0.037，OR=1.67）。Cen AA 区的 KIR 基因在生育男性中明显高于 IVF 男性（p/p=0.0076/0.03，OR=0.67），而 Cen AB+Cen BB 在 IVF 男性中更为常见（p/p=0.0076/0.03，OR=1.50）。我们还观察到 KIR-HLA-C 组合存在有限的关联。
与参与 IVF 的男性相比，生育男性的 KIR 基因和 KIR-HLA-C 组合存在差异。
