Institute of Physical Chemistry, University of Mainz, Duesbergweg 10-14, 55128 Mainz, Germany.
J Phys Chem Lett. 2020 Jun 18;11(12):4554-4558. doi: 10.1021/acs.jpclett.0c01400. Epub 2020 May 28.
We introduce a new approach to monitor the dynamics and spatial patterns of biological molecular assemblies. Our molecular imaging method relies on plasmonic gold nanoparticles as point-like detectors and requires no labeling of the molecules. We show spatial resolution of up to 5 μm and 30 ms temporal resolution, which is comparable to wide-field fluorescence microscopy, while requiring only readily available gold nanoparticles and a dark-field optical microscope. We demonstrate the method on MinDE proteins attaching to and detaching from lipid membranes of different composition for 24 h. We foresee our new imaging method as an indispensable tool in advanced molecular biology and biophysics laboratories around the world.
我们介绍了一种新的方法来监测生物分子组装体的动力学和空间模式。我们的分子成像方法依赖于等离子体金纳米粒子作为点状探测器，并且不需要对分子进行标记。我们展示了高达 5 μm 的空间分辨率和 30 ms 的时间分辨率，这与宽场荧光显微镜相当，而只需要现成的金纳米粒子和暗场光学显微镜。我们在 MinDE 蛋白附着和脱离不同组成的脂质膜 24 小时的实验中证明了该方法的有效性。我们预见我们的新成像方法将成为世界各地先进的分子生物学和生物物理学实验室不可或缺的工具。
