Panteix G, Harf R, de Montclos H, Verdier M F, Gaspar A, Leclercq M
Institut Pasteur Lyon, France.
J Antimicrob Chemother. 1988 Dec;22(6):917-21. doi: 10.1093/jac/22.6.917.
Macrolide antibiotics are concentrated by phagocytic cells in vitro. We studied the in-vivo uptake of josamycin by alveolar macrophages recovered by broncho alveolar lavage performed in patients 3 h after ingestion of 1 g of the drug. Simultaneous determination of the antibiotic levels was performed on the BAL supernatant and the serum and the results compared to those obtained by incubating alveolar macrophages and blood polymorphonuclears at 2 and 8 mg/l. Josamycin concentration was measured using a high pressure liquid chromatography method. Results show that intracellular josamycin levels in vivo are similar to those observed in vitro. Accumulation of the drug also occurs in BAL fluid (reaching about a 100-fold the serum concentration). Pulmonary and serum levels are significantly correlated.
大环内酯类抗生素在体外可被吞噬细胞浓缩。我们研究了在摄入1克药物3小时后，通过支气管肺泡灌洗回收的肺泡巨噬细胞对交沙霉素的体内摄取情况。同时测定支气管肺泡灌洗上清液和血清中的抗生素水平，并将结果与在2毫克/升和8毫克/升浓度下培养肺泡巨噬细胞和血液多形核细胞所获得的结果进行比较。采用高压液相色谱法测定交沙霉素浓度。结果表明，体内细胞内交沙霉素水平与体外观察到的水平相似。药物在支气管肺泡灌洗液中也会蓄积（达到血清浓度的约100倍）。肺部和血清水平显著相关。
