Watanabe H, Katoh H, Ishii M, Komoda Y, Sanda A, Takizawa Y, Ohgi K, Irie M
Department of Microbiology, Hoshi College of Pharmacy, Tokyo.
J Biochem. 1988 Dec;104(6):939-45. doi: 10.1093/oxfordjournals.jbchem.a122587.
The primary structure of a pyrimidine base-specific ribonuclease from bovine brain was determined. The sequence determined is (sequence; see text). Although the sequence homology of this RNase with bovine pancreatic RNase A is 78.2%, it consists of 140 amino acid residues, and it is 16 amino acid residues longer than RNase A at the carboxyl-terminal. In addition to an N-glycosylated long carbohydrate chain, the bovine brain RNase has two short O-glycosylated carbohydrate chains at the 129th and the 133rd serine residues. The additional C-terminal tail of the bovine brain RNase has a unique composition: 6 proline, 5 hydrophobic amino acids, and two basic amino acids, arginine and histidine.
确定了来自牛脑的嘧啶碱基特异性核糖核酸酶的一级结构。所确定的序列为（序列；见正文）。尽管这种核糖核酸酶与牛胰核糖核酸酶A的序列同源性为78.2%，但它由140个氨基酸残基组成，在羧基末端比核糖核酸酶A长16个氨基酸残基。除了一条N-糖基化的长碳水化合物链外，牛脑核糖核酸酶在第129位和第133位丝氨酸残基处还有两条短的O-糖基化碳水化合物链。牛脑核糖核酸酶额外的C末端尾巴具有独特的组成：6个脯氨酸、5个疏水氨基酸以及两个碱性氨基酸，精氨酸和组氨酸。
