Díaz Ruiz Esther, Delgado Bermejo Juan Vicente, León Jurado José Manuel, Navas González Francisco Javier, Arando Arbulu Ander, Fernández-Bolaños Guzmán Juan, Bermúdez Oria Alejandra, González Ariza Antonio
Department of Genetics, Faculty of Veterinary Sciences, University of Córdoba, 14071 Cordoba, Spain.
Institute of Agricultural Research and Training (IFAPA), Alameda del Obispo, 14005 Cordoba, Spain.
Antioxidants (Basel). 2024 Aug 21;13(8):1018. doi: 10.3390/antiox13081018.
Sperm cryopreservation is a fundamental tool for the conservation of avian genetic resources; however, avian spermatozoa are susceptible to this process. To cope with the high production of reactive oxygen species (ROS), the addition of exogenous antioxidants is beneficial. Pectoliv30 is a substance derived from alperujo, and in this study, its effect was analyzed on seminal quality after its addition to the cryopreservation extender of roosters at different concentrations. For this purpose, 16 Utrerana breed roosters were used, and seminal collection was performed in six replicates, creating a pool for each working day with ejaculates of quality. After cryopreservation, one sample per treatment and replicate was thawed, and several seminal quality parameters were evaluated. Statistical analysis revealed numerous correlations between these variables, both positive and negative according to the correlation matrix obtained. Furthermore, the chi-squared automatic interaction detection (CHAID) decision tree (DT) reported significant differences in the hypo-osmotic swelling test (HOST) variable between groups. Moreover, results for this parameter were more desirable at high concentrations of Pectoliv30. The application of this substance extracted from the by-product alperujo as an antioxidant allows the improvement of the post-thawing seminal quality in roosters and facilitates optimization of the cryopreservation process as a way to improve the conservation programs of different endangered poultry breeds.
精子冷冻保存是保护禽类遗传资源的一项基本工具；然而，禽类精子对这一过程很敏感。为了应对活性氧（ROS）的大量产生，添加外源性抗氧化剂是有益的。Pectoliv30是一种源自橄榄果渣的物质，在本研究中，分析了其以不同浓度添加到公鸡冷冻保存稀释液中后对精液质量的影响。为此，使用了16只乌特雷拉纳品种的公鸡，精液采集进行了6次重复，每个工作日将质量合格的射精样本混合成一个样本池。冷冻保存后，每个处理和重复解冻一个样本，并评估几个精液质量参数。统计分析揭示了这些变量之间存在许多相关性，根据得到的相关矩阵，有正有负。此外，卡方自动交互检测（CHAID）决策树（DT）报告了各组之间低渗肿胀试验（HOST）变量存在显著差异。而且，在高浓度Pectoliv30时该参数的结果更理想。将这种从副产品橄榄果渣中提取的物质作为抗氧化剂应用，可以改善公鸡解冻后的精液质量，并有助于优化冷冻保存过程，以此改进不同濒危家禽品种的保护计划。
