Scalia S
Dipartimento di Scienze Farmaceutiche, Università di Ferrara, Italy.
J Chromatogr. 1988 Oct 14;431(2):259-69. doi: 10.1016/s0378-4347(00)83095-9.
A high-performance liquid chromatographic procedure has been developed for the simultaneous assay of the major free bile acids and the corresponding glycine and taurine conjugates in man. No preliminary fractionation into the free, glyco- and tauro-conjugated forms was required. An Ultrasphere ODS column with UV detection at 210 nm and methanol-acetate buffer gradient elution were used. The retention volume of the conjugates was dependent on the sodium acetate concentration in the mobile phase. The method is applicable to the quantification of intragastric bile acids with satisfactory sensitivity, selectivity and precision. Unconjugated and conjugated bile acids present in the gastric juice of patients with bile reflux gastritis were determined directly after Sep-Pak C18 cartridge purification.
已开发出一种高效液相色谱法，用于同时测定人体中的主要游离胆汁酸以及相应的甘氨酸和牛磺酸共轭物。无需预先将其分离为游离、甘氨酸共轭和牛磺酸共轭形式。使用了在210 nm处进行紫外检测的Ultrasphere ODS柱以及甲醇 - 醋酸盐缓冲液梯度洗脱。共轭物的保留体积取决于流动相中醋酸钠的浓度。该方法适用于胃内胆汁酸的定量分析，具有令人满意的灵敏度、选择性和精密度。胆汁反流性胃炎患者胃液中存在的未共轭和共轭胆汁酸，经Sep - Pak C18柱净化后可直接测定。
