Ferguson L R, Rewcastle G W, Lello J M, Alley P G, Seelye R N
Anal Biochem. 1984 Dec;143(2):325-32. doi: 10.1016/0003-2697(84)90671-7.
A method has been developed for extraction and purification of the major bile acids in human feces, and for their quantitative estimation using high-pressure liquid chromatography. Freeze-dried fecal material was extracted with alkaline ethanol and, after removal of neutral steroids, was subjected to thin-layer chromatography, followed by reversed-phase C18 silica cartridge (Sep-Pak) purification. The mixture was further separated into free, and glyco and tauro conjugates by ion-exchange chromatography. Subsequent resolution of individual bile acids was accomplished by HPLC using a counterion pairing method.
已开发出一种从人粪便中提取和纯化主要胆汁酸，并使用高压液相色谱法对其进行定量分析的方法。将冻干的粪便材料用碱性乙醇提取，去除中性类固醇后，进行薄层色谱分析，然后用反相C18硅胶柱（Sep-Pak）纯化。通过离子交换色谱法将混合物进一步分离为游离型、糖基结合型和牛磺结合型共轭物。随后使用反离子配对法通过高效液相色谱法对各个胆汁酸进行分离。
